Home / TGF-beta 2 / TGF-beta 2 Antibody

TGF-beta 2 Antibody

Catalog #: MAB612
10 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB612-SP
MAB612-100
MAB612-500
TGF‑ beta 2 in Human Prostate Cancer.
1 Image
Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

TGF-beta 2 Antibody Summary

Specificity
Detects TGF-beta 2 from multiple species in ELISAs and Western blots. In ELISAs, no cross-reactivity or interfereence with recombinant human (rh) TGF-alpha, recombinant porcineTGF-beta 1, rhTGF-beta 1, recombinant canine TGF-beta 3, recombinant amphibian TGF-beta 5, rhTGF-beta RII, or rhTGF-beta RIII is observed.
Source
Monoclonal Mouse IgG2B Clone # 8607
Purification
Protein A or G purified from ascites
Immunogen
Porcine TGF-beta 1.2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TGF-beta 2 (Catalog # 302-B2)
under non-reducing conditions only
Immunohistochemistry
8-25 µg/mL
See below

Human TGF-beta 2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: TGF‑ beta 2 Biotinylated Antibody (Catalog # BAF302)

Standard: Recombinant Human TGF-beta 2 Protein (Catalog # 302-B2)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry TGF-beta 2 antibody in Human Prostate Cancer by Immunohistochemistry (IHC-P). View Larger

TGF‑ beta 2 in Human Prostate Cancer. TGF-beta 2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-TGF-beta 2 Monoclonal Antibody (Catalog # MAB612) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 2

The active form of TGF-beta 2 is a disulfide-linked homodimer which is generated by activation of the latent TGF-beta 2 complex. TGF-beta s are multifunctional cytokines that activate or repress multiple genes in a wide variety of cell types. Depending on the cell context, TGF-beta can control many cellular processes including growth, morphogenesis, and apoptosis.

Long Name
Transforming Growth Factor beta 2
Entrez Gene IDs
7042 (Human); 21808 (Mouse); 397084 (Porcine)
Alternate Names
BSC-1 cell growth inhibitor; cetermin; Glioblastoma-derived T-cell suppressor factor; G-TSF; MGC116892; polyergin; TGFB2; TGFbeta 2; TGF-beta 2; TGF-beta2; TGF-beta-2; transforming growth factor beta-2; transforming growth factor, beta 2

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for TGF-beta 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. TGF-beta isoforms in cancer: Immunohistochemical expression and Smad-pathway-activity-analysis in thirteen major tumor types with a critical appraisal of antibody specificity and immunohistochemistry assay validity
    Authors: Markus J. Riemenschneider, Maria Hirblinger, Arabel Vollmann-Zwerenz, Peter Hau, Martin A. Proescholdt, Frank Jaschinski et al.
    Oncotarget
  2. Transforming Growth Factor-Beta Produced by Non-small Cell Lung Cancer Cells Contributes to Lung Fibroblast Contractile Phenotype
    Authors: N Kanaji, M Yokohira, N Watanabe, N Kadowaki, S Bandoh
    Anticancer Res., 2018-04-01;38(4):2007-2014.
  3. Early transcriptional pattern of angiogenesis induced by EGCG treatment in cervical tumour cells
    Authors: Oana Tudoran, Olga Soritau, Ovidiu Balacescu, Loredana Balacescu, Cornelia Braicu, Meda Rus et al.
    Journal of Cellular and Molecular Medicine
  4. Insights into homeobox B9: a propeller for metastasis in dormant prostate cancer progenitor cells
    Authors: Y Sui, W Hu, W Zhang, D Li, H Zhu, Q You, R Zhu, Q Yi, T Tang, L Gao, S Zhu, T Yang
    British Journal of Cancer, 2021-07-10;0(0):.
    Species: Xenograft
    Sample Types: Whole Cells
    Applications: Western Blot
  5. Differential TGFbeta pathway targeting by miR-122 in humans and mice affects liver cancer metastasis.
    Authors: Yin S, Fan Y, Zhang H, Zhao Z, Hao Y, Li J, Sun C, Yang J, Yang Z, Yang X, Lu J, Xi J
    Nat Commun, 2016-03-18;7(0):11012.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Potent inhibition of cicatricial contraction in proliferative vitreoretinal diseases by statins.
    Authors: Kawahara S, Hata Y, Kita T, Arita R, Miura M, Nakao S, Mochizuki Y, Enaida H, Kagimoto T, Goto Y, Hafezi-Moghadam A, Ishibashi T
    Diabetes, 2008-07-03;57(10):2784-93.
    Species: Human
    Sample Types: Vitreous Humor
    Applications: Neutralization
  7. Interferon-gamma inhibits transforming growth factor-beta production in human airway epithelial cells by targeting Smads.
    Authors: Wen FQ, Liu X, Kobayashi T, Abe S, Fang Q, Kohyama T, Ertl R, Terasaki Y, Manouilova L, Rennard SI
    Am. J. Respir. Cell Mol. Biol., 2004-01-12;30(6):816-22.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  8. Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells.
    Authors: Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI
    Am. J. Physiol. Lung Cell Mol. Physiol., 2002-05-01;282(5):L1049-56.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. TGF beta 1 and TGF beta 2 proteins in corneas with and without stromal fibrosis: Delayed regeneration of apical epithelial growth factor barrier and the epithelial basement membrane in corneas with stromal fibrosis
    Authors: Carlos de Oliveira R, Tye G, Sampaio L et al.
    Experimental Eye Research
  10. Blood pressure homeostasis is maintained by a P311–TGF-beta axis
    Authors: Kameswara Rao Badri, Ming Yue, Oscar A. Carretero, Sree Latha Aramgam, Jun Cao, Stephen Sharkady et al.
    Journal of Clinical Investigation

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for TGF-beta 2 Antibody

Average Rating: 5 (Based on 2 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used TGF-beta 2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


TGF-beta 2 Antibody
By Anonymous on 11/07/2021
Application: IHC Sample Tested: Skin tissue Species: Human
Immunohistochemistry TGF-beta 2 Antibody MAB612
TGF-beta 2 Antibody
By Anonymous on 03/28/2019
Application: ELISA Sample Tested: Recombinant protein Species: Human

I used this antibody as a capture antibody to generate an in-house ELISA for TGF beta. I coated the plate overnight with the antibody at 2ug/mL in PBS 100uL/well

ELISA TGF-beta 2 Antibody MAB612