Recombinant Rat beta-NGF Protein
Recombinant Rat beta-NGF Protein Summary
Product Specifications
Ser122-Gly241
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
556-NG
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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556-NG/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and NaCl. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
![Recombinant Rat beta-NGF Protein Bioactivity Recombinant Rat beta-NGF Protein Bioactivity](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/protein/beta-NGF_556-NG_666.jpg)
Recombinant Rat beta -NGF (Catalog # 556‑NG) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.1-0.6 ng/mL.
![Recombinant Rat beta-NGF Protein SDS-PAGE Recombinant Rat beta-NGF Protein SDS-PAGE](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/protein/beta-NGF_556-NG_664.jpg)
1 µg/lane of Recombinant Rat beta -NGF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 12 kDa.
Reconstitution Calculator
Background: beta-NGF
NGF was initially isolated in the mouse submandibular gland over three decades ago as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant rat beta -NGF is a homodimer of two 120-amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.
NGF is a well-characterized neurotrophic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor‑dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.
Citations for Recombinant Rat beta-NGF Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Wogonin, a Bioactive Ingredient from Huangqi Guizhi Formula, Alleviates Discogenic Low Back Pain via Suppressing the Overexpressed NGF in Intervertebral Discs
Authors: J Li, W Duan, S Chai, Y Luo, Y Ma, N Yang, M Liu, W He
Mediators of Inflammation, 2023-02-20;2023(0):4436587.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
Neurite Outgrowth-Promoting Compounds from Cockscomb Hydrolysate
Authors: T Koga, A Tai
Nutrients, 2022-03-29;14(7):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
3D micro-organisation printing of mammalian cells to generate biological tissues
Authors: GDM Jeffries, S Xu, T Lobovkina, V Kirejev, F Tusseau, C Gyllensten, AK Singh, P Karila, L Moll, O Orwar
Sci Rep, 2020-11-10;10(1):19529.
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture -
Anti-IL-20 antibody improved motor function and reduced glial scar formation after traumatic spinal cord injury in rats
Authors: JS Lee, YH Hsu, YS Chiu, IM Jou, MS Chang
J Neuroinflammation, 2020-05-14;17(1):156.
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture -
Ameliorative effects of aqueous extract of Forsythiae suspensa fruits on oxaliplatin-induced neurotoxicity in vitro and in vivo
Authors: JM Yi, S Shin, NS Kim, OS Bang
BMC Complement Altern Med, 2019-11-29;19(1):339.
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture -
Preparation and evaluation of microfluidic magnetic alginate microparticles for magnetically templated hydrogels
Authors: I Singh, CS Lacko, Z Zhao, CE Schmidt, C Rinaldi
J Colloid Interface Sci, 2019-11-13;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture -
Neuroprotective Effects of an Aqueous Extract of Forsythia viridissima and Its Major Constituents on Oxaliplatin-Induced Peripheral Neuropathy
Authors: JM Yi, S Shin, NS Kim, OS Bang
Molecules, 2019-03-25;24(6):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Weak power frequency magnetic fields induce microtubule cytoskeleton reorganization depending on the epidermal growth factor receptor and the calcium related signaling.
Authors: Xia Wu, Juan Du, Weitao Song, Meiping Cao, Shude Chen, Ruohong Xia
PLoS ONE, 2018-10-12;0(0):1932-6203.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Differential contributions of peripheral and central mechanisms to pain in a rodent model of osteoarthritis
Authors: AR Haywood, GJ Hathway, V Chapman
Sci Rep, 2018-05-08;8(1):7122.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
Teriflunomide promotes oligodendroglial differentiation and myelination
Authors: P Göttle, A Manousi, D Kremer, L Reiche, HP Hartung, P Küry
J Neuroinflammation, 2018-03-13;15(1):76.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Pterostilbene inhibits reactive oxygen species production and apoptosis in primary spinal cord neurons by activating autophagy via the mechanistic target of rapamycin signaling pathway
Authors: JL He, XH Dong, ZH Li, XY Wang, ZA Fu, N Shen
Mol Med Rep, 2018-01-09;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes
Authors: ZS Nevin, DC Factor, RT Karl, P Douvaras, J Laukka, MS Windrem, SA Goldman, V Fossati, GM Hobson, PJ Tesar
Am. J. Hum. Genet., 2017-03-30;100(4):617-634.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
The TrkA receptor mediates experimental thermal hyperalgesia produced by nerve growth factor: modulation by the p75 neurotrophin receptor
Authors: Gary Strichartz
Neuroscience, 2016-11-05;0(0):.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
Requirement of cAMP signaling for Schwann cell differentiation restricts the onset of myelination.
Authors: Bacallao, Ketty, Monje, Paula V
PLoS ONE, 2015-02-23;10(2):e0116948.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Neuronal exosomes facilitate synaptic pruning by up-regulating complement factors in microglia.
Authors: Bahrini, Insaf, Song, Ji-hoon, Diez, Diego, Hanayama, Rikinari
Sci Rep, 2015-01-23;5(0):7989.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Oligodendroglial maturation is dependent on intracellular protein shuttling.
Authors: Gottle P, Sabo J, Heinen A, Venables G, Torres K, Tzekova N, Parras C, Kremer D, Hartung H, Cate H, Kury P
J Neurosci, 2015-01-21;35(3):906-19.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Incorporation of chitosan microspheres into collagen-chitosan scaffolds for the controlled release of nerve growth factor.
Authors: Zeng, Wen, Rong, Mengyao, Hu, Xueyu, Xiao, Wei, Qi, Fengyu, Huang, Jinghui, Luo, Zhuojing
PLoS ONE, 2014-07-01;9(7):e101300.
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Intermittent hypoxia-induced protein phosphatase 2A activation reduces PC12 cell proliferation and differentiation.
Authors: Chen T, Chiu H, Pan Y, Hsu S, Lin J, Yang K
J Biomed Sci, 2014-05-16;21(0):46.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Opposing roles of PKA and EPAC in the cAMP-dependent regulation of schwann cell proliferation and differentiation [corrected].
Authors: Bacallao, Ketty, Monje, Paula V
PLoS ONE, 2013-12-11;8(12):e82354.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Rab7 silencing prevents mu-opioid receptor lysosomal targeting and rescues opioid responsiveness to strengthen diabetic neuropathic pain therapy.
Authors: Mousa S, Shaqura M, Khalefa B, Zollner C, Schaad L, Schneider J, Shippenberg T, Richter J, Hellweg R, Shakibaei M, Schafer M
Diabetes, 2012-12-10;62(4):1308-19.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
LDL receptor-related protein-1 is a sialic-acid-independent receptor for myelin-associated glycoprotein that functions in neurite outgrowth inhibition by MAG and CNS myelin.
Authors: Stiles, Travis L, Dickendesher, Travis L, Gaultier, Alban, Fernandez-Castaneda, Anthony, Mantuano, Elisabet, Giger, Roman J, Gonias, Steven L
J Cell Sci, 2012-11-06;126(0):209-20.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Contribution of nerve growth factor to augmented TRPV1 responses of muscle sensory neurons by femoral artery occlusion.
Authors: Xing J, Lu J, Li J
Am. J. Physiol. Heart Circ. Physiol., 2009-03-13;296(5):H1380-7.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
Signal transduction mechanism involved in Clostridium perfringens alpha-toxin-induced superoxide anion generation in rabbit neutrophils.
Authors: Oda M, Ikari S, Matsuno T, Morimune Y, Nagahama M, Sakurai J
Infect. Immun., 2006-05-01;74(5):2876-86.
Species: Rabbit
Sample Types: Whole Cells
Applications: Bioassay -
TRPA1 induced in sensory neurons contributes to cold hyperalgesia after inflammation and nerve injury.
Authors: Obata K, Katsura H, Mizushima T, Yamanaka H, Kobayashi K, Dai Y, Fukuoka T, Tokunaga A, Tominaga M, Noguchi K
J. Clin. Invest., 2005-08-18;115(9):2393-401.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Differential activation of MAPK in injured and uninjured DRG neurons following chronic constriction injury of the sciatic nerve in rats.
Authors: Obata K, Yamanaka H, Dai Y, Mizushima T, Fukuoka T, Tokunaga A, Noguchi K
Eur. J. Neurosci., 2004-12-01;20(11):2881-95.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo
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Average Rating: 4.7 (Based on 3 Reviews)
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Reason for Rating: Differentiated PC12 cells with 100ng/mL of NGF for 7 days. Cells look neuronal.