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Recombinant Mouse BMP-7 Protein

Catalog #: 5666-BP
23 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
5666-BP-010/CF

With Carrier

Catalog # Availability Size / Price Qty
5666-BP-010
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (23)
FAQs
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse BMP-7 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.125-0.5 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse BMP-7 protein
Ser292-His430
Accession #
NP_031583
N-terminal Sequence
Analysis
Ser292
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
15.6 kDa (monomer)
SDS-PAGE
16-20 kDa under reducing conditions

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5666-BP (with carrier)

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5666-BP/CF (carrier free)

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COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5666-BP

Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5666-BP/CF

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-7

Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP-1), is a widely expressed TGF-beta superfamily member with important functions during embryogenesis, in the adult, and in disease (1, 2). Mouse BMP-7 is synthesized with a 29 amino acid (aa) signal sequence, a 262 aa propeptide, and a 139 aa growth factor domain (3). The growth factor domain of mouse BMP-7 shares 98% and 100% aa sequence identity with human and rat BMP-7, respectively. The BMP-7 propeptide is cleaved intracellularly but remains in association with the growth factor domain. BMP-7 is subsequently secreted as a tetramer that consists of two propeptides and two disulfide-linked growth factor domains (4, 5). Mature BMP-7 can also form disulfide-linked heterodimers with BMP-2 or BMP-4, complexes that show increased potency and range of activity compared to BMP-7 homodimers (6-8). The presence of the propeptides in the BMP-7 tetramer does not diminish the bioactivity of the growth factor domains (5). Secreted BMP-7 is immobilized in the extracellular matrix as a result of interactions between the propeptide and matrix Fibrillin (4). BMP-7 exerts its biological effects through the type 2 receptors Activin RIIA, Activin RIIB, and BMPR-II and the type 1 receptors Activin RIA, BMPR-IA, and BMPR-IB (2, 5). BMP-7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development (9, 10), inhibits the branching of prostate epithelium (11), and antagonizes epithelial-mesenchymal transition (EMT) (12-14). In pathological conditions, BMP-7 inhibits tumor growth and metastasis (13), ameliorates fibrotic damage in nephritis (12), and promotes neuroregeneration following brain ischemia (15).

References
  1. Carreira, A.C. et al. (2015) J. Dent. Res. 93:335.
  2. Weiskirchen, R. and S.K. Meurer (2013) Front. Biosci. (Landmark Ed.) 18:1407.
  3. Ozkaynak, E. et al. (1991) Biochem. Biophys. Res. Commun. 179:116.
  4. Gregory, K.E. et al. (2005) J. Biol. Chem. 280:27970.
  5. Sengle, G. et al. (2008) J. Mol. Biol. 381:1025.
  6. Israel, D.I. et al. (1996) Growth Factors 13:291.
  7. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  8. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  9. Sampath, T.K. et al. (1992) J. Biol. Chem. 267:20352.
  10. Kazama, I. et al. (2008) J. Am. Soc. Nephrol. 19:2181.
  11. Grishina, I.B. et al. (2005) Dev. Biol. 288:334.
  12. Zeisberg, M. et al. (2003) Nat. Med. 9:964.
  13. Buijs, J.T. et al. (2007) Am. J. Pathol. 171:1047.
  14. Yu, M.-A. et al. (2009) J. Am. Soc. Nephrol. 20:567.
  15. Chou, J. et al. (2006) J. Neurol. Sci. 240:21.
Long Name
Bone Morphogenetic Protein 7
Entrez Gene IDs
655 (Human); 12162 (Mouse); 85272 (Rat)
Alternate Names
BMP7; BMP-7; bone morphogenetic protein 7; Eptotermin alfa; OP-1; OP-1OP1; Osteogenic protein 1

Citations for Recombinant Mouse BMP-7 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Expression of Kielin/chordin-like protein is regulated by BMP-2 in osteoblasts
    Authors: Toba, K;Yamada, A;Sasa, K;Shirota, T;Kamijo, R;
    Bone reports
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Gradients of glucose metabolism regulate morphogen signalling required for specifying tonotopic organisation in the chicken cochlea
    Authors: O'Sullivan, JDB;Blacker, TS;Scott, C;Chang, W;Ahmed, M;Yianni, V;Mann, ZF;
    eLife
    Species: Avian - Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Identification and implication of tissue-enriched ligands in epithelial-endothelial crosstalk during pancreas development
    Authors: M Moulis, SVM Runser, L Glorieux, N Dauguet, C Vanderaa, L Gatto, D Tyteca, P Henriet, FM Spagnoli, D Iber, CE Pierreux
    Scientific Reports, 2022-07-21;12(1):12498.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  4. Factors affecting the induction of uncoupling protein 1 in C2C12 myogenic cells
    Authors: T Yamamoto, Z Diao, M Murakami, F Shimokawa, T Matsui, M Funaba
    Cytokine, 2022-06-20;157(0):155936.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Assessment of BMP responses in an in vitro model of acute ethanol toxicity
    Authors: N Habeeb, S Najafi, JC Perron
    MethodsX, 2022-02-06;9(0):101631.
    Species: N/A
    Sample Types: Recombinant Protein
  6. Offspring production of haploid spermatid-like cells derived from mouse female germline stem cells with chromatin condensation
    Authors: X Hu, H Wang, GG Tian, C Hou, B Xu, X Zhao, Y Zhao, Q Fang, X Li, L He, X Chen, S Li, J Wu
    Cell & bioscience, 2022-01-04;12(1):5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Bone morphogenetic protein 7 promotes resistance to immunotherapy
    Authors: MA Cortez, F Masrorpour, C Ivan, J Zhang, A Younes, Y Lu, MR Estecio, HB Barsoumian, H Menon, MDS Caetano, R Ramapriyan, JE Schoenhals, X Wang, F Skoulidis, MD Wasley, G Calin, P Hwu, JW Welsh
    Nat Commun, 2020-09-24;11(1):4840.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Hyaluronic acid-CD44 interactions promote BMP4/7-dependent Id1/3 expression in melanoma cells
    Authors: RL Wu, G Sedlmeier, L Kyjacova, A Schmaus, J Philipp, W Thiele, BK Garvalov, JP Sleeman
    Sci Rep, 2018-10-08;8(1):14913.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. BMP/SMAD pathway promotes neurogenesis of midbrain dopaminergic neurons in vivo and in human induced pluripotent and neural stem cells
    Authors: VM Jovanovic, A Salti, H Tilleman, K Zega, MM Jukic, H Zou, RH Friedel, N Prakash, S Blaess, F Edenhofer, C Brodski
    J. Neurosci., 2018-01-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Exogenous BMP-7 Facilitates the Recovery of Cardiac Function after Acute Myocardial Infarction through Counteracting TGF-?1 Signaling Pathway
    Authors: Y Jin, X Cheng, J Lu, X Li
    Tohoku J. Exp. Med., 2018-01-01;244(1):1-6.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  11. Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse
    Authors: Y Chen, H Xu, G Lin
    Cell Discov, 2017-12-19;3(0):17046.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. BMP7 dose-dependently stimulates proliferation and cadherin-11 expression via ERK and p38 in a murine metanephric mesenchymal cell line
    Authors: M Awazu, M Nagata, M Hida
    Physiol Rep, 2017-08-01;5(16):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Ca(2+)-associated triphasic pH changes in mitochondria during brown adipocyte activation
    Authors: Y Hou, T Kitaguchi, R Kriszt, YH Tseng, M Raghunath, M Suzuki
    Mol Metab, 2017-05-31;6(8):797-808.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Microglia activation is essential for BMP7-mediated retinal reactive gliosis
    Authors: S Dharmaraja, DL Fisk, CM Sorenson, N Sheibani, TL Belecky-Ad
    J Neuroinflammation, 2017-04-05;14(1):76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells
    Authors: MM Mata-Miran, GJ Vazquez-Za, M Rojas-Lope, V Sanchez-Mo, DG Perez-Ishi, RJ Delgado-Ma
    Biol. Res., 2017-04-04;50(1):14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. The Anti-Osteoanabolic Function of Sclerostin Is Blunted in Mice Carrying a High Bone Mass Mutation of Lrp5.
    Authors: Yorgan T, Peters S, Jeschke A, Benisch P, Jakob F, Amling M, Schinke T
    J Bone Miner Res, 2015-06-08;30(7):1175-83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. A self-avoidance mechanism in patterning of the urinary collecting duct tree.
    Authors: Davies, Jamie A, Hohenstein, Peter, Chang, C-Hong, Berry, Rachel
    BMC Dev Biol, 2014-09-10;14(0):35.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  18. Interactions between BMP-7 and USAG-1 (uterine sensitization-associated gene-1) regulate supernumerary organ formations.
    Authors: Kiso, Honoka, Takahashi, Katsu, Saito, Kazuyuki, Togo, Yumiko, Tsukamoto, Hiroko, Huang, Boyen, Sugai, Manabu, Shimizu, Akira, Tabata, Yasuhiko, Economides, Aris N, Slavkin, Harold C, Bessho, Kazuhisa
    PLoS ONE, 2014-05-09;9(5):e96938.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  19. Neuroprotection by rAAV-mediated gene transfer of bone morphogenic protein 7.
    Authors: Heinonen A, Rahman M, Dogbevia G, Jakobi H, Wolfl S, Sprengel R, Schwaninger M
    BMC Neurosci, 2014-03-11;15(0):38.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Transcription factor-induced lineage programming of noradrenaline and motor neurons from embryonic stem cells.
    Authors: Mong J, Panman L, Alekseenko Z, Kee N, Stanton L, Ericson J, Perlmann T
    Stem Cells, 2014-03-01;32(3):609-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Role for compartmentalization in nephron progenitor differentiation.
    Authors: Brown A, Muthukrishnan S, Guay J, Adams D, Schafer D, Fetting J, Oxburgh L
    Proc Natl Acad Sci U S A, 2013-03-04;110(12):4640-5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs.
    Authors: Chen, Jiekai, Liu, He, Liu, Jing, Qi, Jing, Wei, Bei, Yang, Jiaqi, Liang, Hanquan, Chen, You, Chen, Jing, Wu, Yaran, Guo, Lin, Zhu, Jieying, Zhao, Xiangjie, Peng, Tianran, Zhang, Yixin, Chen, Shen, Li, Xuejia, Li, Dongwei, Wang, Tao, Pei, Duanqing
    Nat Genet, 2012-12-02;45(1):34-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. BAMBI (bone morphogenetic protein and activin membrane-bound inhibitor) reveals the involvement of the transforming growth factor-beta family in pain modulation.
    Authors: Tramullas M, Lantero A, Diaz A, Morchon N, Merino D, Villar A, Buscher D, Merino R, Hurle JM, Izpisua-Belmonte JC, Hurle MA
    J. Neurosci., 2010-01-27;30(4):1502-11.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay

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Recombinant Mouse BMP-7 Protein
By Andres Ocampo on 10/12/2022
Application: In vitro bioactivity in cell culture
Reason for Rating: Protein was easy to dissolve and use. Results were consistent with reported literature.

mBMP7 protein was used to inhibit proliferation of RM-9-CRL-3312 mouse prostate cancer cells. For this, cells were treated with 1 µg/mL for 24 h, and their proliferation using Ki67 ELISA was tested, comparing the cells against untreated cells. A significantly increase in Ki67 expression was observed in the BMP7-treated cells.

In vitro bioactivity in cell culture Recombinant Mouse BMP-7 Protein 5666-BP