Recombinant Mouse BMP-10 Protein

Catalog #: 6038-BP Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
6038-BP-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
6038-BP-025

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse BMP-10 Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED₅₀for this effect is 6-36 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse BMP-10 protein
Asn314-Arg421

Accession #

Q9R229

N-terminal Sequence
Analysis

Asn314

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

12.1 kDa (monomer)

SDS-PAGE

10 kDa, reducing conditions

Product Datasheets

6038-BP (with carrier)

Product Datasheet

COA

SDS

6038-BP/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6038-BP

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 250 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

6038-BP/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 250 μg/mL in 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: BMP-10

Bone morphogenetic protein 10 (BMP-10), along with BMP-9, GDF-5, -6, and -7, belongs to a subgroup of TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors (1-3). Proteolytic removal of the propeptide from the 60 kDa proprotein yields a 12 kDa mature BMP-10 which forms disulfide-linked nonglycosylated homodimers (4, 5). In transfectants, BMP-10 can also be secreted as a proprotein which binds to matrix fibrillin (4, 6). Mature mouse BMP-10 shares 98% and 100% amino acid sequence identity with human and rat BMP-10, respectively, and 48%-64% with mouse BMP-9, GDF-5, -6, and -7. BMP-10 is critical for the proper development of the heart and first appears at the onset of trabeculation and chamber formation (7-9). Homozygous BMP-10 knockout mice die in utero due to arrested cardiac development (8). BMP-10 is required for maintaining expression of the cardiogenic transcription factors NKX2.5 and MEF2C in developing myocardium and promoting the Notch-dependent growth of embryonic cardiomyocytes (8, 10-12). NKX2.5 itself negatively regulates BMP-10 expression in cardiac myocytes (11). BMP-10 in the postnatal heart promotes increased cardiomyocyte and heart size and is upregulated in hypertrophied ventricles (9, 13). Mature BMP-10 accumulates within cardiomyocytes in association with the sarcomere protein Tcap (13). BMP-10 induces signaling through ALK-1, BMPR-IA, BMPR-IB, and BMPR-II in transfectants and non-cardiac cell lines (4, 5). Deletion of BMPR-IA or BMP-10 causes similar cardiac morphogenetic abnormalities (14). In dermal endothelial cells, BMP-10 induces migration, proliferation, and gene expression typically associated with ALK-1 (5).

References