Recombinant Mouse BAMBI/NMA Fc Chimera Protein, CF

Catalog #: 8359-BM Datasheet / COA / SDS

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8359-BM-050

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Recombinant Mouse BAMBI/NMA Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce cell death using Mv1Lu mink lung epithelial cells. The ED₅₀ for this effect is 1-6 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse BAMBI/NMA protein

Mouse BAMBI/NMA (Glu27-Ala152) Accession # Q9D0L6	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q9D0L6

N-terminal Sequence
Analysis

Glu27

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

41 kDa

SDS-PAGE

48-53 kDa, reducing conditions

Product Datasheets

8359-BM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8359-BM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: BAMBI/NMA

BMP and Activin Membrane-Bound Inhibitor (BAMBI), also called Non-Metastatic Gene A (NMA), is a type I transmembrane protein that shares sequence homology with the TGF-beta type I receptor family and functions as a decoy receptor (1). Mouse BAMBI is synthesized as a 260 amino acid (aa) precursor and has a predicted molecular weight of approximately 29 kDa (1). Its extracellular domain contains only one potential N-glycosylation site, and shares 91% and 98% aa sequence identity with the human and rat orthologs, respectively. BAMBI differs from other members of the TGF-beta RI family in that it has a short cytoplasmic region that lacks the intracellular serine/threonine kinase domain (1, 2). It competes with type I receptors to form heterodimers with type II receptors, thus interfering with BMP, Activin, and TGF-beta signaling (2). In fact, BAMBI is believed to be one component of a negative feedback loop for BMP signaling that serves to increase the dynamic signaling range for BMPs (3, 4). During development, BAMBI is prominently expressed in gastrulation, neurulation, and during the development of teeth and bones, and is often co-expressed with other BMP family members (1-5). BAMBI is also ubiquitously expressed in the adult, with the highest levels being observed in heart, lung, spleen, kidney, bladder, and testes (6). BAMBI expression is induced by TGF-beta and Wnt signaling (7, 8). It cooperates with inhibitory Smad6 and Smad7 to inhibit TGF-beta signaling (9). In contrast, BAMBI promotes Wnt signaling via its interactions with Frizzled receptors (10). BAMBI has been shown to modulate various processes including adipogenesis, thrombus formation and stability, cell proliferation, and opioid signaling in neuropathic pain (10-13). Additionally, aberrant BAMBI expression is believed to be a factor in the development of cancer, inflammation, and fibrotic processes (7, 14-18).

References

Knight, C. et al. (2001) J. Dent. Res. 80:1895.
Onichtchouk, D. et al. (1999) Nature 401:480.
Paulsen, M. et al. (2011) Proc. Natl. Acad. Sci. USA 108:10202.
Montero, J.A. et al. (2008) Dev. Biol. 321:343.
Grotewold, L. et al. (2001) Mech. Dev. 100:327.
Lakoski, K. et al. (2003) Endocrinology 144:4180.
Sekiya, T. et al. (2004) J. Biol. Chem. 279:6840.
Sekiya, T. et al. (2004) Biochem. Biophys. Res. Commun. 320:680.
Yan, X. et al. (2009) J. Biol. Chem. 284:30097.
Lin, Z. et al. (2008) J. Biol. Chem. 283:33053.
Luo, X. et al. (2012) Diabetes 61:124.
Salles-Crawley, I.I. et al. (2014) Blood 123:2873.
Lantero, A. et al. (2014) J. Neurosci. 34:5385.
Seki, E. et al. (2007) Nat. Med. 13:1324.
Khin, S.S. et al. (2009) Int. J. Cancer 125:328.
Fritzmann, J. et al. (2009) Gastroenterology 137:165.
Drömann, D. et al. (2010) Respir. Res. 11:67.
Li, Y.S. et al. (2013) PLoS One 8:e76289.

Long Name

BMP and Activin Membrane-bound Inhibitor

Entrez Gene IDs

25805 (Human); 68010 (Mouse)

Alternate Names

BAMBI; BMP and activin membrane-bound inhibitor homolog (Xenopus laevis); BMP and activin membrane-bound inhibitor homolog; NMA; NMANon-metastatic gene A protein; Putative transmembrane protein NMA