Recombinant Human TrkC Fc Chimera Protein

Catalog #: 373-TC
6 Citations Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
373-TC-050/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
373-TC-050

R&D Systems Recombinant Proteins and Enzymes

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Product Details

Citations (6)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TrkC Fc Chimera Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit NT-3-induced proliferation of BaF‑TrkB‑BD mouse pro‑B cells transfected with TrkB. The ED₅₀ for this effect is 0.05-0.2 µg/mL in the presence of 40 ng/mL of rhNT-3.

Source

Mouse myeloma cell line, NS0-derived human TrkC protein

Human TrkC (Cys32 - Asp428) Accession # Q96CY4	DIEGRMD	Human IgG₁ (Pro100 - Lys330)	6-His tag
N-terminus			C-terminus

Accession #

Q96CY4

N-terminal Sequence
Analysis

Cys32

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

72 kDa (monomer)

SDS-PAGE

130-150 kDa, reducing conditions

Product Datasheets

373-TC (with carrier)

Product Datasheet

COA

SDS

373-TC/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

373-TC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

373-TC/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: TrkC

The neurotrophins, including NGF, BDNF, NT-3 and NT-4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). P75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low-affinity to transduce cellular signaling pathways that synergize or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT-3, TrkB binds BDNF, NT-3 and NT-4/5, and TrkC only binds NT-3 (1 - 2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, Trks are highly conserved between species with the extracellular domains of human and mouse TrkC's showing 94% amino acid sequence identity (5). The proteins also exhibit cross-species activity. The primary location of TrkC expression is in the nervous system and, specifically, in regions of the CNS. Low level TrkC expression has also been observed in a wide variety of tissues outside the nervous system (6).

References

Huang, E.J. and L.F. Reichardt. (2003) Annu. Rev. Biochem. 72:(epub ahead of print).
Dechant, G. (2001) Cell Tissue Res. 305:229.
Schneider, R. and M. Schweiger (1991) Oncogene 6:1807.
Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
Menn, B. et al. (1998) J. Comp. Neurol. 401:47.
Shelton, D. et al. (1995) J. Neurosci. 15:477.

Long Name

Neurotrophic Tyrosine Kinase Receptor C

Entrez Gene IDs

4916 (Human); 18213 (Mouse); 29613 (Rat)

Alternate Names

EC 2.7.10; EC 2.7.10.1; ETS related protein-neurotrophic receptor tyrosine kinase fusion protein; ETV6-NTRK3 fusion; gp145(trkC); GP145-TrkC; Neurotrophic tyrosine kinase receptor type 3; neurotrophic tyrosine kinase, receptor, type 3; NT 3 receptor; NT-3 growth factor receptor; NTRK3; TrkC tyrosine kinase; TrkC; trk-C; TRKCneurotrophin 3 receptor; tyrosine kinase receptor C

Related Research Areas

Citations for Recombinant Human TrkC Fc Chimera Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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BDNF/NT3 Regulate AIS Location and Affect Neuronal Excitability in Cultured Hippocampal Neurons
Authors: Y Guo, ZJ Su, YK Chen, Z Chai
J. Neurochem., 2017-05-26;0(0):.
Species: Rat
Sample Types: Cell Culture Supernates
Applications: Bioassay
Trk receptor signaling and sensory neuron fate are perturbed in human neuropathy caused by Gars mutations
Authors: JN Sleigh, JM Dawes, SJ West, N Wei, EL Spaulding, A Gómez-Mart, Q Zhang, RW Burgess, MZ Cader, K Talbot, XL Yang, DL Bennett, G Schiavo
Proc. Natl. Acad. Sci. U.S.A, 2017-03-28;0(0):.
Applications: Bioassay
Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling.
Authors: Vaegter CB, Jansen P, Fjorback AW
Nat. Neurosci., 2010-11-21;14(1):54-61.
Applications: Surface Plasmon Resonance
Neurotrophin-3 production promotes human neuroblastoma cell survival by inhibiting TrkC-induced apoptosis.
Authors: Bouzas-Rodriguez J, Cabrera JR, Delloye-Bourgeois C, Ichim G, Delcros JG, Raquin MA, Rousseau R, Combaret V, Benard J, Tauszig-Delamasure S, Mehlen P
J. Clin. Invest., 2010-02-15;120(3):850-8.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi.
Authors: Lu B, PereiraPerrin M
Infect. Immun., 2008-06-09;76(8):3530-8.
Species: Bacteria
Sample Types: Trypanosoma
Applications: PcIP
Rapid axoglial signaling mediated by neuregulin and neurotrophic factors.
Authors: Esper RM, Loeb JA
J. Neurosci., 2004-07-07;24(27):6218-27.
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay