Recombinant Human Pleiotrophin/PTN Protein, CF Summary
Product Specifications
Gly33-Asp168
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
252-PL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Pleiotrophin/PTN
PTN was identified independently by several groups as a novel heparin-binding, developmentally regulated cytokine. Depending on the biological activities studied, this protein has variously been referred to as heparin-binding brain mitogen (HBBM), heparin-binding growth factor-8 (HBGF-8), heparin-binding neurite promoting factor, heparin-binding neurotrophic factor (HBNF), heparin-affinity regulatory peptide (HARP), heparin-binding growth-associated molecule (HB-GAM), osteoblast-specific factor (OSF-1), and pleiotrophin. PTN is a highly conserved protein; the amino acid sequences of human, bovine, rat, and mouse PTN share > 98% homology.
PTN is a member of a family of heparin-binding proteins that share sequence, structural, and functional similarity. Other members of this family include midkine (MK), and chicken retinoic acid-induced heparin-binding protein (RI-HB), an avian homologue of MK. The expression of all these cytokines is restricted and highly regulated during development.
PTN can be used as an attachment substrate to stimulate neurite outgrowth in mixed cultures of embryonic rat, mouse or chicken brain cells. Although both natural and recombinant human PTN have been reported to be mitogenic for fibroblasts, endothelial, and epithelial cells, the data are still highly controversial. The insect cell-derived recombinant PTN preparations produced at R&D Systems lack mitogenic activities when tested on various fibroblast, endothelial, and epithelial cell lines. PTN has been shown to transform NIH-3T3 and SW-13 cells, as evidenced by anchorage-independent growth and tumor formation in the nude mouse. These results suggest that, in spite of the conflicting reports of PTN’s growth-promoting activity in vitro, PTN may have a role in abnormal cell growth in vivo.
Citations for Recombinant Human Pleiotrophin/PTN Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Harnessing developmental dynamics of spinal cord extracellular matrix improves regenerative potential of spinal cord organoids
Authors: Sun, Z;Chen, Z;Yin, M;Wu, X;Guo, B;Cheng, X;Quan, R;Sun, Y;Zhang, Q;Fan, Y;Jin, C;Yin, Y;Hou, X;Liu, W;Shu, M;Xue, X;Shi, Y;Chen, B;Xiao, Z;Dai, J;Zhao, Y;
Cell stem cell
Species: Rabbit, Rat
Sample Types: Organoid
Applications: Bioassay -
Unique structural characteristics and biological activities of heparan sulfate isolated from the mantle of the scallop Chlamys farreri
Authors: Qin, Y;Xu, Y;Yi, H;Shi, L;Wang, X;Wang, W;Li, F;
Carbohydrate polymers
Species: N/A
Sample Types: Carbohydrates
Applications: Surface Plasmon Resonance -
Dissecting the spermatogonial stem cell niche using spatial transcriptomics
Authors: Rajachandran, S;Zhang, X;Cao, Q;Caldeira-Brant, AL;Zhang, X;Song, Y;Evans, M;Bukulmez, O;Grow, EJ;Nagano, M;Orwig, KE;Chen, H;
Cell reports
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Pleiotrophin-Loaded Mesoporous Silica Nanoparticles as a Possible Treatment for Osteoporosis
Authors: D Lozano, B Leiva, IS Gómez-Esca, S Portal-Núñ, AR de Górtaza, M Manzano, M Vallet-Reg
Pharmaceutics, 2023-02-16;15(2):.
Species: Human
Sample Types: Protein
Applications: Bioassay -
Discovery of PTN as a serum-based biomarker of pro-metastatic prostate cancer
Authors: S Liu, M Shen, EC Hsu, CA Zhang, F Garcia-Mar, R Nolley, K Koul, MA Rice, M Aslan, SJ Pitteri, C Massie, A George, JD Brooks, VJ Gnanapraga, T Stoyanova
British Journal of Cancer, 2020-12-08;0(0):.
Species: N/A
Sample Types: Recombinant Protein
Applications: ELISA Standard -
A glucocorticoid- and diet-responsive pathway toggles adipocyte precursor cell activity in vivo.
Authors: Wong J, Krueger K, Costa M, Aggarwal A, Du H, McLaughlin T, Feldman B
Sci Signal, 2016-10-25;9(451):ra103.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Receptor-type protein-tyrosine phosphatase zeta is a functional receptor for interleukin-34.
Authors: Nandi S, Cioce M, Yeung Y, Nieves E, Tesfa L, Lin H, Hsu A, Halenbeck R, Cheng H, Gokhan S, Mehler M, Stanley E
J Biol Chem, 2013-06-06;288(30):21972-86.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Pleiotrophin produced by multiple myeloma induces transdifferentiation of monocytes into vascular endothelial cells: a novel mechanism of tumor-induced vasculogenesis.
Authors: Chen H, Campbell RA, Chang Y, Li M, Wang CS, Li J, Sanchez E, Share M, Steinberg J, Berenson A, Shalitin D, Zeng Z, Gui D, Perez-Pinera P, Berenson RJ, Said J, Bonavida B, Deuel TF, Berenson JR
Blood, 2008-12-05;113(9):1992-2002.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Pleiotrophin is highly expressed by myeloma cells and promotes myeloma tumor growth.
Authors: Chen H, Gordon MS, Campbell RA, Li M, Wang CS, Lee HJ, Sanchez E, Manyak SJ, Gui D, Shalitin D, Said J, Chang Y, Deuel TF, Baritaki S, Bonavida B, Berenson JR
Blood, 2007-03-16;110(1):287-95.
Applications: ELISA (Standard)
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