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Recombinant Human BMP-8b Protein, CF

Catalog #: 9316-BP
3 Citations Datasheet / COA / SDS
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9316-BP-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BMP-8b Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the cell growth of DU145 human prostate carcinoma cells. Miyazaki, H. et al. (2004) Oncogene 23:9326. The ED50 for this effect is 0.7-3.5 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human BMP-8b protein
Ala264-His402
Accession #
P34820
N-terminal Sequence
Analysis
Ala264
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
16 kDa
SDS-PAGE
17-20 kDa, reducing conditions

Product Datasheets

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9316-BP

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9316-BP

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 250 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-8b

BMP-8, also known as osteogenic protein 2 (OP-2), was first isolated from a hippocampal library in a screen to identify relatives of BMP-7/OP-1 (1). BMPs are a family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta ) superfamily. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis. Cellular responses to BMPs are mediated by hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors (2-4). BMP-8a and BMP-8b, produced from separate genes, share 98% aa sequence identity in human but only 74% in mouse within the mature regions. Human BMP-8b is synthesized as a 402 aa precursor protein that is cleaved between Arg263 and Ala264 to release the C-terminal mature protein. Mature human BMP-8b shares 91% and 71% aa sequence identity with mouse mature BMP-8a and -8b, respectively. BMP-8a is expressed during pregnancy in the deciduum and trophoblast cells, by inner root sheath cells of developing hair follicles, and by the epididymis and spermatids (5-7). In the mouse it cooperates with BMP-7 in the maintenance of spermatogenesis but is not required for the initiation of spermatogenesis (7, 8). BMP-8b, in contrast, is required for both the initiation and maintenance of spermatogenesis (9). BMP-8a is induced during osteoblast differentiation at the onset of mineralization and during the osteogenic phase of bone repair in osteoblasts and osteocytes (10-12). BMP-8b is also highly expressed in osteosarcomas and pancreatic cancer, and it contributes to tumor progression (13, 14). BMP-8b is also expressed in adipose tissue and the hypothalamus where it contributes to the regulation of energy balance and thermogenesis (15, 16).

References
  1. Ozkaynak, E. et al. (1992) J. Biol. Chem. 267:25220.
  2. Chen, D. et al. (2004) Growth Factors 22:233.
  3. Bragdon, B. et al. (2010) Cell Signal. Oct 16 epub.
  4. Singh, A. and R.J. Morris (2010) Cytokine Growth Factor Rev. 21:299.
  5. Zhao, G.-Q. and B.L. Hogan (1996) Mech. Dev. 57:159.
  6. Ying, Y. and G.-Q. Zhao (2000) Biol. Reprod. 63:1781.
  7. Zhao, G.-Q. et al. (1998) Development 125:1103.
  8. Zhao, G.-Q. et al. (2001) Dev. Biol. 240:212.
  9. Zhao, G.-Q. et al. (1998) Genes Dev. 10:1657.
  10. van der Horst, G. et al. (2002) Bone 31:661.
  11. Cho, T.-J. et al. (2002) J. Bone Miner. Res. 17:513.
  12. Paic, F. et al. (2009) Bone 45:682.
  13. Sulzbacher, I. et al. (2002) J. Clin. Pathol. 55:381.
  14. Cheng, Z. et al. (2014) Oncol. Rep. 32:1861.
  15. Whittle, A.J. et al. (2012) Cell 149:871.
  16. Martins, L. et al. (2016) Cell Rep. 16:2231.
Long Name
Bone Morphogenetic Protein 8b
Entrez Gene IDs
656 (Human); 12164 (Mouse)
Alternate Names
BMP-8; BMP-8b; bone morphogenetic protein 8b; MGC131757; OP-3

Citations for Recombinant Human BMP-8b Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Regulation of both transcription and RNA turnover contribute to germline specification
    Authors: K Tan, MF Wilkinson
    Oncogene, 2022-07-22;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Obesity induces resistance to central action of BMP8B through a mechanism involving the BBSome
    Authors: E Rial-Pensa, O Freire-Agu, M Ríos, DF Guo, C Contreras, P Seoane-Col, S Tovar, R Nogueiras, C Diéguez, K Rahmouni, M López
    Molecular Metabolism, 2022-02-23;0(0):101465.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Production of viable chicken by allogeneic transplantation of primordial germ cells induced from somatic cells
    Authors: R Zhao, Q Zuo, X Yuan, K Jin, J Jin, Y Ding, C Zhang, T Li, J Jiang, J Li, M Zhang, X Shi, H Sun, Y Zhang, Q Xu, G Chang, Z Zhao, B Li, X Wu, Y Zhang, J Song, G Chen, B Li
    Nature Communications, 2021-05-20;12(1):2989.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay

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