Recombinant Human beta-NGF Protein Summary
Product Specifications
Ser122-Ala241
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
256-GF
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetic Acid with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
256-GF/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetic Acid. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
![Recombinant Human beta-NGF Protein Bioactivity Recombinant Human beta-NGF Protein Bioactivity](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/protein/beta-NGF_256-GF_322.jpg)
Recombinant Human beta -NGF (Catalog # 256-GF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.2‑2 ng/mL.
![Recombinant Human beta-NGF Protein SDS-PAGE Recombinant Human beta-NGF Protein SDS-PAGE](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/protein/beta-NGF_256-GF_321.jpg)
1 µg/lane of Recombinant Human beta -NGF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Reconstitution Calculator
Background: beta-NGF
NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.
NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor‑dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.
Citations for Recombinant Human beta-NGF Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
51
Citations: Showing 1 - 10
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iPSC motor neurons, but not other derived cell types, capture gene expression changes in postmortem sporadic ALS motor neurons
Authors: Held, A;Adler, M;Marques, C;Reyes, CJ;Kavuturu, AS;Quadros, ARAA;Ndayambaje, IS;Lara, E;Ward, M;Lagier-Tourenne, C;Wainger, BJ;
Cell reports
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
An LRP1-binding motif in cellular prion protein replicates cell-signaling activities of the full-length protein
Authors: Mantuano, E;Zampieri, C;Azmoon, P;Gunner, CB;Heye, KR;Gonias, SL;
JCI insight
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Stem cell-nanomedicine system as a theranostic bio-gadolinium agent for targeted neutron capture cancer therapy
Authors: YH Lai, CY Su, HW Cheng, CY Chu, LB Jeng, CS Chiang, WC Shyu, SY Chen
Nature Communications, 2023-01-18;14(1):285.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay
Authors: S Nyström, A Kendall, S Adepu, A Lindahl, E Skiöldebra
Research in Veterinary Science, 2022-08-22;151(0):156-163.
Species: Horse
Sample Types: Cell Lysates
Applications: Control -
Specific Attenuation of Purinergic Signaling during Bortezomib-Induced Peripheral Neuropathy In Vitro
Authors: AK Holzer, I Suciu, C Karreman, T Goj, M Leist
International Journal of Molecular Sciences, 2022-03-29;23(7):.
Species: Human
Sample Types: Whole Cells
Applications: Differentiation, Differentiation -
Tmem160 contributes to the establishment of discrete nerve injury-induced pain behaviors in male mice
Authors: D Segelcke, HK Fischer, M Hütte, S Dennerlein, F Benseler, N Brose, EM Pogatzki-Z, M Schmidt
Cell Reports, 2021-12-21;37(12):110152.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
TLR4 Associated Signaling Disrupters as a New Means to Overcome HERV-W Envelope-Mediated Myelination Deficits
Authors: P Göttle, K Schichel, L Reiche, L Werner, A Zink, A Prigione, P Küry
Frontiers in Cellular Neuroscience, 2021-11-23;15(0):777542.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
TrkA Interacts with and Phosphorylates STAT3 to Enhance Gene Transcription and Promote Breast Cancer Stem Cells in Triple-Negative and HER2-Enriched Breast Cancers
Authors: AT Regua, NR Aguayo, SA Jalboush, DL Doheny, SG Manore, D Zhu, GL Wong, A Arrigo, CJ Wagner, Y Yu, A Thomas, MD Chan, J Ruiz, G Jin, R Strowd, P Sun, J Lin, HW Lo
Cancers, 2021-05-12;13(10):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Regulation of host and virus genes by neuronal miR-138 favours herpes simplex virus 1 latency
Authors: B Sun, X Yang, F Hou, X Yu, Q Wang, HS Oh, P Raja, JM Pesola, EAH Vanni, S McCarron, J Morris-Lov, AHM Ng, GM Church, DM Knipe, DM Coen, D Pan
Nature Microbiology, 2021-02-08;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Modulation of Human Adipose Stem Cells' Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro
Authors: KM Prautsch, A Schmidt, V Paradiso, DJ Schaefer, R Guzman, DF Kalbermatt, S Madduri
Cells, 2020-08-21;9(9):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay, Cell Culture -
Mechanical stimulation of Schwann cells promote peripheral nerve regeneration via extracellular vesicle-mediated transfer of microRNA 23b-3p
Authors: B Xia, J Gao, S Li, L Huang, L Zhu, T Ma, L Zhao, Y Yang, K Luo, X Shi, L Mei, H Zhang, Y Zheng, L Lu, Z Luo, J Huang
Theranostics, 2020-07-11;10(20):8974-8995.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay, Cell Culture -
Establishment of a simple one-step method for oligodendrocyte progenitor cell preparation from rodent brains
Authors: A Yoshida, K Takashima, T Shimonaga, M Kadokura, S Nagase, S Koda
J. Neurosci. Methods, 2020-05-30;0(0):108798.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Generation of Multipotent Stem Cells from Adult Human Peripheral Blood Following the Treatment with Platelet-Derived Mitochondria
Authors: H Yu, W Hu, X Song, Y Zhao
Cells, 2020-05-29;9(6):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
An in vivo platform to select and evolve aggregation-resistant proteins
Authors: JS Ebo, JC Saunders, PWA Devine, AM Gordon, AS Warwick, B Schiffrin, SE Chin, E England, JD Button, C Lloyd, NJ Bond, AE Ashcroft, SE Radford, DC Lowe, DJ Brockwell
Nat Commun, 2020-04-14;11(1):1816.
Species: Human
Sample Types: Protein
Applications: Bioassay -
Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells
Authors: AR Nickolls, MM Lee, DF Espinoza, M Szczot, RM Lam, Q Wang, J Beers, J Zou, MQ Nguyen, HJ Solinski, AA AlJanahi, KR Johnson, ME Ward, AT Chesler, CG Bönnemann
Cell Rep, 2020-01-21;30(3):932-946.e7.
Species: Human
Sample Types: Whole Cells
Applications: Differentiation, Differentiation -
Pharmacological Properties of JTE-952, an Orally Available and Selective Colony Stimulating Factor 1 Receptor Kinase Inhibitor
Authors: N Uesato, N Miyagawa, K Inagaki, R Kakefuda, Y Kitagawa, Y Matsuo, T Yamaguchi, T Hata, K Ikegashira, M Matsushita
Biol. Pharm. Bull., 2020-01-01;43(2):325-333.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Extracellular Vesicles Derived From Olfactory Ensheathing Cells Promote Peripheral Nerve Regeneration in Rats
Authors: B Xia, J Gao, S Li, L Huang, T Ma, L Zhao, Y Yang, J Huang, Z Luo
Front Cell Neurosci, 2019-12-06;13(0):548.
Species: Rat
Sample Types: Whole Tissue
Applications: Bioassay -
Promotion of Axon Growth by the Secreted End of a Transcription Factor
Authors: EP McCurdy, KM Chung, CR Benitez-Ag, U Hengst
Cell Rep, 2019-10-08;29(2):363-377.e5.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
No evidence for altered plasma NGF and BDNF levels in fibromyalgia patients
Authors: D Baumeister, W Eich, S Saft, O Geisel, R Hellweg, A Finn, CI Svensson, J Tesarz
Sci Rep, 2019-09-20;9(1):13667.
Species: Human
Sample Types: Plasma
Applications: ELISA Standard -
Differentiation of Motor Neuron-Like Cells from Tonsil-Derived Mesenchymal Stem Cells and Their Possible Application to Neuromuscular Junction Formation
Authors: S Park, JY Kim, S Myung, N Jung, Y Choi, SC Jung
Int J Mol Sci, 2019-06-01;20(11):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Inhibition of Tropomyosin Receptor Kinase A Signaling Negatively Regulates Megakaryopoiesis and induces Thrombopoiesis
Authors: A Kizilyer, MV Singh, VB Singh, S Suwunnakor, J Palis, SB Maggirwar
Sci Rep, 2019-02-26;9(1):2781.
Species: Human
Sample Types:
Applications: Bioassay -
A C-terminal cysteine residue is required for peptide-based inhibition of the NGF/TrkA interaction at nM concentrations: implications for peptide-based analgesics
Authors: AJ Poole, L Frigotto, ME Smith, C Baar, G Ivanova-Be, A Jaulent, C Stace, CG Ullman, AV Hine
Sci Rep, 2019-01-30;9(1):930.
Species: Human
Sample Types: Peptide, Recombinant Protein
Applications: ELISA (Capture), ELISA (Standard) -
Modeling Diabetic Corneal Neuropathy in a 3D In Vitro Cornea System
Authors: PM Deardorff, TB McKay, S Wang, CE Ghezzi, DM Cairns, RD Abbott, JL Funderburg, KR Kenyon, DL Kaplan
Sci Rep, 2018-11-23;8(1):17294.
Species: Human
Sample Types: Complex Sample Type
Applications: Bioassay -
Efficient derivation of sympathetic neurons from human pluripotent stem cells with a defined condition
Authors: K Kirino, T Nakahata, T Taguchi, MK Saito
Sci Rep, 2018-08-27;8(1):12865.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Potent and rapid activation of tropomyosin-receptor kinase A in endometrial stromal fibroblasts by seminal plasma
Authors: JW Martin, JC Chen, J Neidleman, K Tatsumi, J Hu, LC Giudice, WC Greene, NR Roan
Biol. Reprod., 2018-08-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Integrative proteomic and transcriptomic analysis provides evidence for TrkB (NTRK2) as a therapeutic target in combination with tyrosine kinase inhibitors for non-small cell lung cancer
Authors: DR Gomez, LA Byers, M Nilsson, L Diao, J Wang, L Li, P Tong, M Hofstad, B Saigal, I Wistuba, N Kalhor, S Swisher, Y Fan, WK Hong, M Suraokar, C Behrens, C Moran, JV Heymach
Oncotarget, 2018-01-30;9(18):14268-14284.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Functional effects of TrkA inhibition on system xC--mediated glutamate release and cancer-induced bone pain
Authors: T Miladinovi, RG Ungard, K Linher-Mel, S Popovic, G Singh
Mol Pain, 2018-01-01;14(0):1744806918776.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Immunohistochemical and immunocytochemical study of mechanoreceptors in anterior cruciate ligament reconstruction with the remnant-preserving technique using Achilles tendon allografts
Authors: KC Chun, SH Lee, JW Kim, EJ Jin, KM Kim, CH Chun
J Orthop Surg Res, 2017-06-14;12(1):93.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Engineering the surface properties of a human monoclonal antibody prevents self-association and rapid clearance in vivo
Sci Rep, 2016-12-20;6(0):38644.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Hierarchical CRMP2 posttranslational modifications control NaV1.7 function
Proc. Natl. Acad. Sci. U.S.A, 2016-12-08;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Nerve Growth Factor Attenuates Apoptosis and Inflammation in the Diabetic Cornea
Invest. Ophthalmol. Vis. Sci, 2016-12-01;57(15):6767-6775.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
BMS-708163 and Nilotinib restore synaptic dysfunction in human embryonic stem cell-derived Alzheimer's disease models
Sci Rep, 2016-09-19;6(0):33427.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A novel inhibitor of p75-neurotrophin receptor improves functional outcomes in two models of traumatic brain injury
Authors: S Delbary-Go, S Lee, M Baroni, I Lamarche, M Arnone, B Canolle, A Lin, J Sacramento, EA Salegio, MN Castel, N Delesque-T, A Alam, P Laboudie, B Ferzaz, P Savi, JM Herbert, GT Manley, AR Ferguson, JC Bresnahan, F Bono, MS Beattie
Brain, 2016-04-15;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Isolation, expansion and neural differentiation of stem cells from human plucked hair: a further step towards autologous nerve recovery
Authors: Margriet A Huisman
Cytotechnology, 2015-12-24;68(5):1849-58.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Effects of Reducing Suppressors of Cytokine Signaling-3 (SOCS3) Expression on Dendritic Outgrowth and Demyelination after Spinal Cord Injury.
Authors: Park K, Lin C, Li K, Lee Y
PLoS ONE, 2015-09-18;10(9):e0138301.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Sensory neurons do not induce motor neuron loss in a human stem cell model of spinal muscular atrophy.
Authors: Schwab, Andrew J, Ebert, Allison
PLoS ONE, 2014-07-23;9(7):e103112.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
2, 3, 7, 8-Tetrachlorodibenzo-P-dioxin (TCDD) induces premature senescence in human and rodent neuronal cells via ROS-dependent mechanisms.
Authors: Wan C, Liu J, Nie X, Zhao J, Zhou S, Duan Z, Tang C, Liang L, Xu G
PLoS ONE, 2014-02-24;9(2):e89811.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Canine epidermal neural crest stem cells: characterization and potential as therapy candidate for a large animal model of spinal cord injury.
Authors: Gericota B, Anderson J, Mitchell G, Borjesson D, Sturges B, Nolta J, Sieber-Blum M
Stem Cells Transl Med, 2014-01-17;3(3):334-45.
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay -
Neuropeptides released from trigeminal neurons promote the stratification of human corneal epithelial cells.
Authors: Ko J, Mizuno Y, Ohki C, Chikama T, Sonoda K, Kiuchi Y
Invest Ophthalmol Vis Sci, 2014-01-07;55(1):125-33.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Humanization of antibodies using heavy chain complementarity-determining region 3 grafting coupled with in vitro somatic hypermutation.
Authors: Bowers P, Neben T, Tomlinson G, Dalton J, Altobell L, Zhang X, Macomber J, Wu B, Toobian R, McConnell A, Verdino P, Chau B, Horlick R, King D
J Biol Chem, 2013-01-25;288(11):7688-96.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
EGF and bFGF pre-treatment enhances neural specification and the response to neuronal commitment of MIAMI cells.
Authors: Delcroix GJ, Curtis KM, Schiller PC, Montero-Menei CN
Differentiation, 2010-09-01;80(4):213-27.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Long-term delivery of nerve growth factor by encapsulated cell biodelivery in the Gottingen minipig basal forebrain.
Authors: Fjord-Larsen L, Kusk P, Tornoe J, Juliusson B, Torp M, Bjarkam CR, Nielsen MS, Handberg A, Sorensen JC, Wahlberg LU
Mol. Ther., 2010-07-27;18(12):2164-72.
Species: Porcine, Rat
Sample Types: Whole Cells
Applications: Bioassay -
A multiplex immunoassay for human adipokine profiling.
Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clin. Chem., 2010-06-08;56(0):1320.
Applications: ELISA (Standard) -
Down-regulation of the met receptor tyrosine kinase by presenilin-dependent regulated intramembrane proteolysis.
Authors: Foveau B, Ancot F, Leroy C, Petrelli A, Reiss K, Vingtdeux V, Giordano S, Fafeur V, Tulasne D
Mol. Biol. Cell, 2009-03-18;20(9):2495-507.
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay -
Neurotrophins modulate monocyte chemotaxis without affecting macrophage function.
Authors: Samah B, Porcheray F, Gras G
Clin. Exp. Immunol., 2008-01-08;151(3):476-86.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Effects of NGF and BDNF on baseline glutamate and dopamine release in the hippocampal formation of the adult rat.
Authors: Paredes D, Granholm AC, Bickford PC
Brain Res., 2007-01-12;1141(0):56-64.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
RhoA-kinase coordinates F-actin organization and myosin II activity during semaphorin-3A-induced axon retraction.
Authors: Gallo G
J. Cell. Sci., 2006-08-15;119(0):3413-23.
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay -
Elicitation of immunity to HIV type 1 Gag is determined by Gag structure.
Authors: Young KR, Ross TM
AIDS Res. Hum. Retroviruses, 2006-01-01;22(1):99-108.
Applications: Western Blot -
Nerve growth factor expression by PLG-mediated lipofection.
Authors: Whittlesey KJ, Shea LD
Biomaterials, 2005-11-28;27(11):2477-86.
Applications: Western Blot -
Growth cones turn and migrate up an immobilized gradient of the laminin IKVAV peptide.
Authors: Adams DN, Kao EY, Hypolite CL, Distefano MD, Hu WS, Letourneau PC
J. Neurobiol., 2005-01-01;62(1):134-47.
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay -
Nerve growth factor overexpression and autocrine loop in breast cancer cells.
Authors: Dolle L, El Yazidi-Belkoura I, Adriaenssens E, Nurcombe V, Hondermarck H
Oncogene, 2003-08-28;22(36):5592-601.
Species: Chicken
Sample Types: N/A, Whole Cells
Applications: Bioassay, ELISA (Standard)
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