Recombinant Cynomolgus/Rhesus Glypican 3 His-tag Protein, CF

Catalog #: 10509-GP Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10509-GP-050

Recombinant Cynomolgus/Rhesus Glypican 3 His-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus/Rhesus Glypican 3 His-tag Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human FGF basic/FGF2 (146aa) (Catalog # 233-FB/CF) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Glypican 3 His-tag (Catalog # 10509-GP) binds with an ED₅₀ of 0.075-0.45 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived Glypican 3 protein
Gln25-Asn554, with a C-terminal 6-His tag

Accession #

XP_005594665.1

N-terminal Sequence
Analysis

Gln25 (blocked) & Ser359

Predicted Molecular Mass

38 kDa (alpha chain), 23 kDa (beta chain), 61 kDa (full length)

SDS-PAGE

65-90 kDa, under non-reducing conditions

Product Datasheets

10509-GP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10509-GP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human FGF basic/FGF2 (146 aa) Protein (233-FB/CF) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Glypican 3 His-tag Protein (10509-GP) binds with an ED₅₀ of 0.075-0.45 μg/mL.

Reconstitution Calculator

Background: Glypican 3

Glypican 3 (GPC3), also known as GTR2-2, intestinal protein OCI-5 and MXR7, is a member of the heparan sulfate (HS) proteoglycan family (1). In mammals, six glypican family members have been identified, all sharing a conserved core protein that is linked to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor. GPC3 can be cleaved by furin to produce two subunits that are linked by disulfide bonds: a 40 kDa N-terminal alpha subunit that can be secreted into the blood and a 30 kDa membrane-bound C-terminal beta subunit containing two HS glycan chains (1-3). Several isoforms of GPC3 due to alternative splicing have been reported (1). Within the mature extracellular domain, cynomolgus/rhesus GPC3 shares 99% amino acid sequence identity with human GPC3. GPC3 is an important biomarker present in the serum of hepatocellular carcinoma patients, which distinguishes benign from cancerous nodules (4). Glypican 3 is over-expressed in hepatocellular carcinomas and binding of GPC3 to CD81 promotes development of carcinomas by activation of Hippo pathways in hepatocytes (5). Our Avi-tag Biotinylated Recombinant Glypican 3 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Ho, M. and Kim, H. (2012) Eur. J. Cancer. 47:333.
Haruyama, Y. and Kataoka, H. (2016) World J. Gastroenterol. 22:275.
Shimizu, Y. et al. (2019) Front Oncol. 9:248.
Ge, S. et al. (2018) Filmus, Int J Clin Exp Pathol. 11(12):5774.
Xue, Y. et al. (2018) Am J Pathol. 188(6):1469.

Entrez Gene IDs

2719 (Human); 14734 (Mouse); 25236 (Rat); 102137748 (Cynomolgus Monkey)

Alternate Names

DGSX; Glypican 3; glypican proteoglycan 3; glypican-3; GPC3; GTR2-2; heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; OCI5; OCI-5; secreted glypican-3; SGB; SGBS; SGBS1SDYS