Rat CXCR7/RDC-1 Antibody

Catalog #: MAB8399 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8399
MAB8399-SP

Detection of CXCR7/RDC‑1 in HEK293 Human Cell Line Transfected with Rat CXCR7/RDC-1 and eGFP by Flow Cytometry.

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All CXCR7/RDC-1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat CXCR7/RDC-1 Antibody Summary

Species Reactivity

Rat

Specificity

Stains rat CXCR7/RDC-1 transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2A Clone # 896032

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with rat CXCR7/RDC-1
Met1-Lys362
Accession # NP_445804

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CXCR7/RDC‑1 in HEK293 Human Cell Line Transfected with Rat CXCR7/RDC-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) rat CXCR7/RDC-1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Rat CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB8399) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR7/RDC-1

CXCR7 (CXC Chemokine Receptor 7), also known as GPRN1, RDC-1 and Chemokine Orphan Receptor 1, is a 60 kDa member of the G-protein coupled receptor 1 family. It is expressed on multiple cell types, including neurons, T cells, NK cells, neutrophils, B cells plus angiogenic endothelial cells. CXCR7 forms both homodimers and heterodimers with CXCR4. It selectively binds I-TAC and SDF-1, and appears to involve beta -Arrestin 2 during signaling. Notably, a CXCR7:CXCR4 heterodimer shows increased responsiveness to SDF-1, and I-TAC may actually block some SDF-1-mediated migration activity.

Entrez Gene IDs

57007 (Human); 12778 (Mouse); 84348 (Rat)

Alternate Names

ACKR3; chemokine (C-X-C motif) receptor 7; Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7; CMKOR1; CXCR7; CXC-R7; CXCR-7; G protein-coupled receptor; GPR159; GPR159G-protein coupled receptor 159; RDC-1; RDC1G-protein coupled receptor RDC1 homolog