Rat CXCR3 Antibody

Catalog #: MAB8109 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8109
MAB8109-SP

Detection of CXCR3 in CHO Chinese Hamster Cell Line Transfected with Rat CXCR3 and eGFP by Flow Cytometry.

1 Image

All CXCR3 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat CXCR3 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CXCR3 in ELISA. Stains rat CXCR3 transfected cells but not irrelevant transfectants by Flow Cytometry.

Source

Monoclonal Mouse IgG2b Clone # 868013

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CXCR3 in CHO Chinese Hamster Cell Line Transfected with Rat CXCR3 and eGFP by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with rat CXCR3 and eGFP was stained with either (A) Mouse Anti-Rat CXCR3 Monoclonal Antibody (Catalog # MAB8109) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: CXCR3

CXCR3, also known as CD183, is an approximately 41 kDa (predicted) 7-transmembrane spanning receptor for the chemokines CXCL9, CXCL10, and CXCL11. It is expressed on activated T cells, B cells, and NK cells during inflammation. It is additionally upregulated on solid tumor cells, tumor endothelium, and cancer stem cells. CXCR3 plays a role in leukocyte recruitment to sites of inflammation. It also contributes to Th1 biased responses during autoimune diseases rheumatoid arthritis, systemic erythematosis, and type 1 diabetes. Rat CXCR3 shares 86% and 96% amino acid sequence identity with human and mouse CXCR3, respectively.

Entrez Gene IDs

2833 (Human); 12766 (Mouse); 84475 (Rat)

Alternate Names

CD183 antigen; CD183; chemokine (C-X-C motif) receptor 3; chemokine (C-X-C) receptor 3; CKR-L2IP-10 receptor; CMKAR3; C-X-C chemokine receptor type 3; CXCR3; CXC-R3; CXCR-3; G protein-coupled receptor 9CD182; GPR9; GPR9Mig-R; Interferon-inducible protein 10 receptor; IP10 receptor; IP10-R; Mig receptor; MigR