Rat CD25/IL-2R alpha PE-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: CD25/IL-2R alpha

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I transmembrane (TM) glycoprotein that functionally belongs to a family of cytokine receptors that utilize the common gamma chain subunit ( gamma _c) (1-3). IL-2 R alpha is expressed on activated T cells, and on regulatory T cells (Treg), mast cells, fibroblasts, NK cells, endthelial cells and pre-B cells (4-6). The rat IL-2 R alpha cDNA encodes a 267 amino acid (aa) precursor that includes a 21 aa signal peptide, a 214 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and an 11 aa cytoplasmic domain (7). Within the ECD, rat IL-2 R alpha shares 58% and 81% aa sequence identity with human and mouse IL-2 R alpha, respectively. It shares approximately 15% aa sequence identity with IL-4, -7, -9, -15, and -21 receptor subunits that also complex with gamma _c. By itself, IL-2 R alpha binds IL-2 with low affinity. It then associates with IL-2 R beta and subsequently to gamma _c to generate a ternary high affinity IL-2 receptor complex. Notably, it would appear that IL-2 R alpha also plays a role in the formation of supramolecular membrane complexes that also include MHC-II molecules and additional gamma _c -related alpha -chains (8).  A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (9, 10). Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders (11). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (12, 13). IL-2 R alpha is also required for the development of CD4⁺CD25⁺ Treg which suppress autoreactive CD4⁺ T cells, thereby contributing to peripheral T cell homeostasis (12-15).

Product Datasheets

Related Research Areas

• Acute Lymphoblastic Leukemia
• Acute Myeloid Leukemia
• B Cell Activation
• B Cell Markers
• Cancer Biomarkers
• Common gamma Chain Receptor Family
• Cytokines and Receptors in Allergy and Asthma
• Cytokines and Receptors in Tfh Cell Differentiation and Function
• Group 1 ILCs
• Group 2 ILCs
• Isolation, Culture, and Identification of Natural Killer Cells
• LTi Cells
• Lymphoma
• NCR+ILC3
• NCR-ILC3
• Preeclampsia
• Receptors in the Jak/STAT Pathway
• Regulatory T Cells (Tregs)
• T Cell Cytokine Signaling