Rat B7-2/CD86 Antibody Summary
Met1-Ile250
Accession # NP_064466
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![IL‑2 Secretion Induced by B7‑2/CD86 and Neutralization by Rat B7‑2/CD86 Antibody. IL‑2 Secretion Induced by B7‑2/CD86 and Neutralization by Rat B7‑2/CD86 Antibody.](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/B7-2_MAB13401_Block_Neutralize_9022.jpg)
IL‑2 Secretion Induced by B7‑2/CD86 and Neutralization by Rat B7‑2/CD86 Antibody. Recombinant Rat B7-2/CD86 Fc Chimera (Catalog # 1340-B2) co-stimulates IL-2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL-2 Quantikine ELISA Kit (Catalog # D2050). IL-2 secretion elicited by Recombinant Rat B7-2/CD86 Fc Chimera (1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat B7-2/CD86 Monoclonal Antibody (Catalog # MAB13401). The ND50 is typically 0.3-1.0 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B7-2/CD86
For optimal T cell expansion and activation, a signal induced by the engagement of the T cell receptor and a “co-stimulatory” signal(s) through distinct T cell surface molecules are required. Members of the B7 superfamily of counter-receptors were identified by their ability to interact with co-stimulatory molecules found on the surface of T cells. Members of the B7 superfamily are type I membrane proteins and include B7-1 (CD80), B7-2 (CD86), B7-H1 (PD-L1), B7-H2 (B7RP-1), B7-H3, and PD-L2 (1). B7-2 is expressed constitutively at low levels on most Antigen Presenting Cells (APC) and is rapidly upregulated upon cell activation (2). T cells express two different receptors (CD28 and CTLA-4) capable of binding both B7-1 and B7-2 (2). B7-2 binds to CD28 with the low affinity but binds to CTLA-4 with intermediate affinity. In contrast, B7-1 binds CD28 with intermediate affinity and CTLA-4 with high affinity. Additionally, these molecules have different kinetics for binding CD28 and CTLA-4 with B7-2 having a higher-binding dissociation kinetics (1). Engagement of CD28 by B7-2 increases T cell proliferation and IL-2, IL-4, and IFN-gamma production, thereby enhancing the immune response (3). In contrast, engagement of CTLA-4 is involved in the down-regulation of the immune response (4). Rat B7-2 cDNA encodes a 313 amino acid (aa) precursor protein containing a an extracellular domain, a transmembrane domain, and a cytoplasmic domain. Rat and human B7-1 share 54% aa identity.
- Coyle, A.J. and J-C. Gutierrez-Ramos (2001) Nature Immunol. 2:203.
- Sharpe, A.H. and G.J. Freeman (2002) Nature Reviews 2:116.
- Freeman, G.J. et al. (1995) Immunity 5:523.
- Walunas, T.L. et al. (1994) Immunity 1:405.
Product Datasheets
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