Rabbit IgG PE-conjugated Antibody

Catalog # Availability Size / Price Qty
F0110
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Detection of Rabbit IgG Primary Antibody by Flow Cytometry
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Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (2)

Rabbit IgG PE-conjugated Antibody Summary

Species Reactivity
Rabbit
Specificity
Rabbit IgG specific IgG was purified by rabbit IgG affinity chromatography.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Rabbit IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of rabbit origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Rabbit IgG Primary Antibody by Flow Cytometry View Larger

Detection of Rabbit IgG Primary Antibody by Flow Cytometry Mouse splenocytes were stained with Rat Anti-Mouse CD161/NK1.1 APC-conjugated Monoclonal Antibody (FAB7614A) and either (A) Rabbit Anti-Mouse KLRG1 Monoclonal Antibody (MAB6944) or (B) Normal Rabbit IgG Control (AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) at 10 µL/106 cells.

Flow Cytometry View Larger

Detection of Rabbit IgG Primary Antibody by Flow Cytometry U937 cells were stained with Rabbit Anti-Human IFN alpha beta R1 Monoclonal Antibody (2452, filled histogram) or (B) Normal Rabbit IgG Control (AB-105-C, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0110) at 10 µL/106 cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitution default not known
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Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

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Citations for Rabbit IgG PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. High-dimensional analysis reveals an immune atlas and novel neutrophil clusters in the lungs of model animals with Actinobacillus pleuropneumoniae-induced pneumonia
    Authors: Li, N;Zhu, J;Chen, P;Bao, C;Wang, J;Abdelaal, T;Chen, D;Zhu, S;Wang, W;Mao, J;Scicluna, BP;Koning, F;Li, F;Lei, L;
    Veterinary research
    Species: Mouse
    Sample Types: Whole Cell
  2. Dietary Supplementation with Biobran/MGN-3 Increases Innate Resistance and Reduces the Incidence of Influenza-like Illnesses in Elderly Subjects: A Randomized, Double-Blind, Placebo-Controlled Pilot Clinical Trial
    Authors: AF Elsaid, S Agrawal, A Agrawal, M Ghoneum
    Nutrients, 2021-11-19;13(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Cellular and humoral peritoneal immunity to Mesocestoides vogae metacestode infection in mice
    Authors: T Ma?ak Kuba, D Mudro?ová, M Vargová, K Reiterová, G Hr?ková
    Parasites & vectors, 2021-01-18;14(1):54.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Plasma neutrophil extracellular trap level is modified by disease severity and inhaled corticosteroids in chronic inflammatory lung diseases
    Authors: Z Gál, A Gézsi, É Pállinger, T Visnovitz, A Nagy, A Kiss, M Sultész, Z Csoma, L Tamási, G Gálffy, C Szalai
    Sci Rep, 2020-03-09;10(1):4320.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Integrins alpha4beta1 and alphaVbeta3 are Reduced in Endothelial Progenitor Cells from Diabetic Dyslipidemic Mice and May Represent New Targets for Therapy in Aortic Valve Disease
    Authors: A Filippi, A Constantin, N Alexandru, G Voicu, CA Constantin, D Rebleanu, M Fenyo, D Simionescu, A Simionescu, I Manduteanu, A Georgescu
    Cell Transplant, 2020-01-01;29(0):9636897209462.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. A stable and reproducible human blood-brain barrier model derived from hematopoietic stem cells.
    Authors: Cecchelli R, Aday S, Sevin E, Almeida C, Culot M, Dehouck L, Coisne C, Engelhardt B, Dehouck M, Ferreira L
    PLoS ONE, 2014-06-17;9(6):e99733.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Bone marrow fibroblasts parallel multiple myeloma progression in patients and mice: in vitro and in vivo studies.
    Authors: Frassanito M, Rao L, Moschetta M, Ria R, di Marzo L, De Luisi A, Racanelli V, Catacchio I, Berardi S, Basile A, Menu E, Ruggieri S, Nico B, Ribatti D, Fumarulo R, Dammacco F, Vanderkerken K, Vacca A
    2013-09-02;0(0):.
    Species: Human, Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC, IHC-Fr
  8. High Amount of Transcription Factor IRF8 Engages AP1-IRF Composite Elements in Enhancers to Direct Type 1 Conventional Dendritic Cell Identity
    Authors: Sunkyung Kim, Prachi Bagadia, David A. Anderson, Tian-Tian Liu, Xiao Huang, Derek J. Theisen et al.
    Immunity

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Reviews for Rabbit IgG PE-conjugated Antibody

Average Rating: 5 (Based on 2 Reviews)

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Rabbit IgG PE-conjugated Antibody
By Danlee Enzler on 05/01/2018
Application: ELISA Sample Tested: Serum Species: Rabbit

Rabbit IgG PE-conjugated Antibody
By Anonymous on 03/24/2017
Application: Flow Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human

Flow cytometry analysis of MDA-MB-231 cells incubated with Rabbit anti-Human NFKB p65 antibody (red) or Isotype Control IgG (black), followed by 10 uL/106 cells of PE-conjugated Goat Anti-Rabbit secondary antibody (#F0110).