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Rabbit IgG Fluorescein-conjugated Antibody

Catalog #: F0112
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
F0112
Detection of Rabbit IgG Primary Antibody by Flow Cytometry
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Product Details
Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rabbit IgG Fluorescein-conjugated Antibody Summary

Species Reactivity
Rabbit
Specificity
Rabbit IgG specific IgG was purified by rabbit IgG affinity chromatography.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Rabbit IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of rabbit origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Rabbit IgG Primary Antibody by Flow Cytometry View Larger

Detection of Rabbit IgG Primary Antibody by Flow Cytometry Daudi human Burkitt's lymphoma cell line untreated (open histogram) or treated (filled histogram) was stained with Rabbit Anti-Human Phospho-STAT2 (Y689) Monoclonal Antibody (Catalog # MAB2890), followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Rabbit IgG Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Co-Treatment with Human Leukocyte Extract and Albendazole Stimulates Drug's Efficacy and Th1 Biased Immune Response in Mesocestoides vogae (Cestoda) Infection via Modulation of Transcription Factors, Macrophage Polarization, and Cytokine Profiles
    Authors: G Hr?ková, T Ma?ak Kuba, D Mudro?ová, Z Jur?acková, D Ciglanová
    Pharmaceutics, 2023-02-06;15(2):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Targeting stanniocalcin-1-expressing tumor cells elicits efficient antitumor effects in a mouse model of human lung cancer
    Authors: K Abe, M Kanehira, S Ohkouchi, S Kumata, Y Suzuki, H Oishi, M Noda, A Sakurada, E Miyauchi, T Fujiwara, H Harigae, Y Okada
    Cancer Medicine, 2021-04-07;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Cellular and humoral peritoneal immunity to Mesocestoides vogae metacestode infection in mice
    Authors: T Ma?ak Kuba, D Mudro?ová, M Vargová, K Reiterová, G Hr?ková
    Parasites & vectors, 2021-01-18;14(1):54.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. CD147 promotes the proliferation, invasiveness, migration and angiogenesis of human lung carcinoma cells
    Authors: S Yang, F Qi, C Tang, H Wang, H Qin, X Li, J Li, W Wang, C Zhao, H Gao
    Oncol Lett, 2016-12-14;13(2):898-904.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Tacrolimus Protects Podocytes from Injury in Lupus Nephritis Partly by Stabilizing the Cytoskeleton and Inhibiting Podocyte Apoptosis.
    Authors: Liao R, Liu Q, Zheng Z, Fan J, Peng W, Kong Q, He H, Yang S, Chen W, Tang X, Yu X
    PLoS ONE, 2015-07-10;10(7):e0132724.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Complement c5a receptor facilitates cancer metastasis by altering T-cell responses in the metastatic niche.
    Authors: Vadrevu S, Chintala N, Sharma S, Sharma P, Cleveland C, Riediger L, Manne S, Fairlie D, Gorczyca W, Almanza O, Karbowniczek M, Markiewski M
    2014-05-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  7. The novel receptor tyrosine kinase Axl is constitutively active in B-cell chronic lymphocytic leukemia and acts as a docking site of nonreceptor kinases: implications for therapy.
    Authors: Ghosh AK, Secreto C, Boysen J, Sassoon T, Shanafelt TD, Mukhopadhyay D, Kay NE
    Blood, 2010-12-06;117(6):1928-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Leptin receptor expression and signaling in lymphocytes: kinetics during lymphocyte activation, role in lymphocyte survival, and response to high fat diet in mice.
    Authors: Papathanassoglou E, El-Haschimi K, Li XC, Matarese G, Strom T, Mantzoros C
    J. Immunol., 2006-06-15;176(12):7745-52.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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