Porcine CD31/PECAM-1 Antibody

Catalog #: MAB3387 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3387
MAB3387-SP

All CD31/PECAM-1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Porcine CD31/PECAM-1 Antibody Summary

Species Reactivity

Porcine

Specificity

Detects porcine CD31/PECAM‑1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) CD31, rhCECAM-1, rhICAM-1, rhICAM-2, rhICAM-3, rhVCAM-1, recombinant mouse (rm) MadCAM-1, rmCD31, or rmICAM-5 is observed.

Source

Monoclonal Rat IgG_2A Clone # 377521

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant porcine CD31/PECAM‑1
Gln28-Lys602 (predicted)
Accession # Q95242

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Porcine CD31/PECAM‑1 (Catalog # 3387-PC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD31/PECAM-1

CD31, also known as PECAM-1 (platelet-endothelial cell adhesion molecule-1), is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to the vascular system, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. CD31 is concentrated at cell-cell junctions and is required for transendothelial migration (TEM) (1 - 3). The extracellular domain (ECD) of CD31 has ten potential N-glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains immunoregulatory tyrosine-based inhibitory and switch motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum CD31 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length CD31 is predominant. A form lacking the ITSM predominates in mouse (9). Porcine CD31 ECD shows 74%, 73%, 70%, 63% and 62% amino acid (aa) identity with bovine, canine, human, mouse and rat CD31, respectively. CD31 participates with other adhesion molecules for most functions but is the critical molecule for TEM. Homotypic CD31 adhesion in trans combined with cycling of CD31 to and from surface-connected endothelial cell vesicles leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. CD31^-/- mice are deficient in chemokine-mediated chemotaxis (14).

References

Ilan, N. and J.A. Madri (2003) Curr Opin. Cell Biol. 15:515.
Nasu, K. et al. (1999) Transplantation 68:861.
Liao, F. et al. (1997) J. Exp. Med. 185:1349.
Nakada, M.T. et al. (2000) J. Immunol. 164:452.
Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
Ilan, N. et al. (2001) FASEB J. 15:362.
Eugenin, E.A. et al. (2006)J. Leukoc. Biol. 79:444.
Losy, J. et al. (1999) 99:169.
Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
Mamdouh, Z. et al. (2003) Nature 421:748.
Gao, C. et al. (2003) Blood 102:169.
Falati, S. et al. (2006) Blood 107:535.
Wee, J. L. and D.E. Jackson (2005) Blood 106:3816.
Wu, Y. et al. (2005) J. Immunol. 175:3484.

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Entrez Gene IDs

5175 (Human); 18613 (Mouse); 29583 (Rat)

Alternate Names

adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule