PJ 34 hydrochloride

Biological Activity

PJ 34 hydrochloride is a potent inhibitor of poly(ADP-ribose) polymerase (PARP) (EC₅₀ = 20 nM). ~1000-fold more potent than 3-Aminobenzamide (Cat. No. 0788). Protects primary neuronal cells from oxygen-glucose deprivation in vitro and reduces infarct size following focal cerebral ischemia in vivo. Displays protective effects against cisplatin-induced kidney injury. Also displays activity at Pim-1 and Pim-2 kinases at higher concentrations (IC₅₀ values are 3.7 and 16 μM respectively).

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. Identification of Pim kinases as novel targets for PJ34 with confounding effects in PARP biology.
   Antolin et al.
   ACS Chem.Biol., 2012;7:1962
2. Protective effects of PJ34, a novel, potent inhibitor of poly(ADP-ribose) polymerase (PARP) in in vitro and in vivo models of stroke.
   Abdelkarim et al.
   Int.J.Mol.Med., 2001;7:255
3. Poly(ADPR)polymerase inhibition and apoptosis induction in cDDP-treated human carcinoma cell lines.
   Gambi et al.
   Biochem.Pharmacol., 2008;75:2356
4. Poly(ADP-ribose) polymerase 1 activation is required for cisp. nephrotoxicity.
   Kim et al.
   Kidney Int., 2012;82:193

Product Datasheets