NucA nuclease Antibody

Catalog #: MAB100633 Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB100633-100
MAB100633-SP
Detection of NucA nuclease by Western Blot.
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NucA nuclease Antibody Summary

Specificity
Detects NucA nuclease in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG2B Clone # 1028905
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived s. marcescens NucA nuclease
Met1-Asn266
Accession # P13717
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
Recombinant NucA nuclease Protein
Simple Western
10 µg/mL
Recombinant NucA nuclease Protein

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot NucA nuclease View Larger

Detection of NucA nuclease by Western Blot. Western blot shows recombinant NucA nuclease protein. PVDF membrane was probed with 2 µg/mL of Mouse Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100633) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for NucA nuclease at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western Detection of NucA nuclease by Simple Western View Larger

Detection of NucA nuclease by Simple WesternTM. Simple Western lane view shows recombinant NucA nuclease protein, loaded at 0.2 mg/mL. A specific band was detected for NucA nuclease at approximately 35 kDa (as indicated) using 10 µg/mL of Mouse Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100633). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NucA nuclease

Long Name
NucA nuclease
Entrez Gene IDs
23387471 (Bacterial)
Alternate Names
DcD; NucA nuclease

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