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Neuron-specific beta-III Tubulin Biotinylated Antibody

Catalog #: BAM1195
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAM1195
beta -III Tubulin in Rat Cortical Stem Cells.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Neuron-specific beta-III Tubulin Biotinylated Antibody Summary

Specificity
Detects mammalian and chicken neuron-specific  beta -III tubulin but not other beta -tubulin isotypes.
Source
Monoclonal Mouse IgG2A Clone # TuJ-1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Rat brain-derived microtubules
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
beta -III Tubulin
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry beta -III Tubulin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

beta -III Tubulin in Rat Cortical Stem Cells. beta -III Tubulin was detected in immersion fixed 7 day differentiated rat cortical stem cells using Neuron-specific beta -III Tubulin Biotinylated Monoclonal Antibody (Catalog # BAM1195) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-III Tubulin

Class III beta tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of class III beta -tubulin has been suggested to be one of the earliest markers to signal neuronal commitment in primitive neuroepithelium (1, 2).

References
  1. Caccamo, D. et al. (1989) Lab. Invest. 60:390.
  2. Alexander, J. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4685.
Entrez Gene IDs
10381 (Human)
Alternate Names
Beta3-tubulin; beta-4; betaIII Tubulin; beta-III Tubulin; beta-Tubulin III; Class III beta-tubulin; TUBB3; TUBB4 CFEOM3A; Tubulin Beta 3; tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4; Tubulin beta-III; tubulin, beta 3; TUJ1 antigen; TUJ-1 antigen

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Citations for Neuron-specific beta-III Tubulin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. The Hippo pathway effector YAP is an essential regulator of ductal progenitor patterning in the mouse submandibular gland
    Authors: Aleksander D Szymaniak, Rongjuan Mi, Shannon E McCarthy, Adam C Gower, Taylor L Reynolds, Michael Mingueneau et al.
    eLife
  2. Smooth Muscle Differentiation Is Essential for Airway Size, Tracheal Cartilage Segmentation, but Dispensable for Epithelial Branching
    Authors: Randee E. Young, Mary-Kayt Jones, Elizabeth A. Hines, Rongbo Li, Yongfeng Luo, Wei Shi et al.
    Developmental Cell
  3. Mast cell-derived neurotrophin 4 mediates allergen-induced airway hyperinnervation in early life
    Mucosal Immunol, 2016-02-10;9(6):1466-1476.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-P
  4. An NT4/TrkB-dependent increase in innervation links early-life allergen exposure to persistent airway hyperreactivity.
    Authors: Aven, Linh, Paez-Cortez, Jesus, Achey, Rebecca, Krishnan, Ramaswam, Ram-Mohan, Sumati, Cruikshank, William, Fine, Alan, Ai, Xingbin
    FASEB J, 2013-11-12;28(2):897-907.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy
    Authors: Claudia Prahst, Parham Ashrafzadeh, Thomas Mead, Ana Figueiredo, Karen Chang, Douglas Richardson et al.
    eLife
  6. A Shh/miR-206/BDNF Cascade Coordinates Innervation and Formation of Airway Smooth Muscle
    Authors: Kelsi Radzikinas, Linh Aven, Zhihua Jiang, Thanh Tran, Jesus Paez-Cortez, Karthik Boppidi et al.
    The Journal of Neuroscience

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