Mouse ZIP4H/Tex11 Antibody

Catalog #: AF5627 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5627
AF5627-SP

Detection of Mouse ZIP4H/Tex11 by Western Blot.

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All ZIP4H/Tex11 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse ZIP4H/Tex11 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse ZIP4H/Tex11 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse ZIP4H/Tex11
Met768-Leu947
Accession # Q14AT2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse ZIP4H/Tex11 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line either mock transfected, transfected with human ZIP4H, or transfected with mouse ZIP4H. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse ZIP4H/Tex11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5627) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ZIP4H/Tex11 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZIP4H/Tex11

ZIP4 homolog (ZIP4H), also known as testis expressed sequence 11 (Tex11) is a 947 amino acid (aa) X chromosome-encoded protein that is selectively expressed in primordial male germ cells. It associates with Nbs1 of the Mre11 protein complex which results in localization to areas of synaptonemal contact between meiotic chromosomes. During meiosis, ZIP4H promotes sister chromatid synapsis and crossover in response to programmed double stranded DNA breaks. ZIP4H deficiency leads to increased spermatocyte apoptosis and male sterility. Alternate splicing in mouse generates an additional isoform with a substitution (aa 691‑707) and truncation (aa 708‑947) at the C-terminus. Within aa 768‑947, mouse ZIP4H shares 44% and 73% aa sequence identity with human and rat ZIP4H, respectively.

Long Name

Zinc Transporter Protein 4 Homolog

Entrez Gene IDs

56159 (Human); 83558 (Mouse); 501588 (Rat)

Alternate Names

testis expressed 11; testis expressed sequence 11; testis-expressed sequence 11 protein; Tex11; TGC1; TSGA3; ZIP4H