Mouse Wnt-11 Antibody

Name: Mouse Wnt-11 Antibody
Brand: R&D Systems
SKU: MAB3746
Rating: 4.5 (2 reviews)

Catalog #: MAB3746 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3746
MAB3746-SP

Detection of Mouse Wnt‑11 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Wnt-11 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Wnt-11 in direct ELISAs and Western blots. In these formats, this antibody shows no cross-reactivity with recombinant human Wnt-2, -7a, -7b, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, -8b, or –9a. Western blotting with human cell extracts demonstrates that this antibody also detects human Wnt-11.

Source

Monoclonal Rat IgG₁ Clone # 310622

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Wnt-11
Leu39-Ala79 and Ser225-Arg297
Accession # NP_033545

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Wnt‑11 by Western Blot. Western blot shows lysates of mouse embryo (13 d.p.c.) tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Wnt-11 Monoclonal Antibody (Catalog # MAB3746) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt-11 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Wnt‑11 in Mouse Embryo. Wnt-11 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rat Anti-Mouse Wnt-11 Monoclonal Antibody (Catalog # MAB3746) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-11

Wnt-11 belongs to the Wnt family of secreted, highly conserved, cysteine-rich signaling glcoproteins that are important in vertebrate development. Mouse Wnt-11a is expressed in early development and is required for cardiogenesis and branching morphogenesis of ureters. Wnt-11 signals by a non-canonical pathway and may repress canonical signaling by other Wnts. Mouse and human Wnt‑11 shares 96% identity over the sequences expressed in the immunogen.

Long Name

Wingless-type MMTV Integration Site Family, Member 11

Entrez Gene IDs

7481 (Human); 22411 (Mouse)

Alternate Names

HWNT11; MGC141946; MGC141948; protein Wnt-11; wingless-type MMTV integration site family, member 11; Wnt11; Wnt-11