Mouse VLDLR Biotinylated Antibody Summary
Thr25-Ala798
Accession # AAA59384
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VLDLR
VLDL R is a 105 kDa type I integral membrane protein that belongs to the LDL receptor family. It plays a significant role in lipid metabolism and in nervous system development and function (1, 2). Mouse VLDL R has a 770 amino acid (aa) extracellular domain (ECD) and a 54 aa cytoplasmic region. The ECD contains eight LDLR class A repeats, three EGF-like repeats, six LDLR class B repeats, and a juxtamembrane region that is rich in O-linked glycosylation (3, 4). The cytoplasmic domain contains one NPXY internalization motif. VLDL R is predominantly expressed in striated muscle, adipose tissue, brain, and endothelial cells lining capillaries and small arterioles (3-6). VLDL R participates in the tissue uptake of fatty acids from plasma by mediating the internalization of ApoE-containing lipoparticles (i.e. VLDL, beta ‑VLDL, and chylomicron remnants) (5, 7). VLDL R binds and internalizes lipoprotein lipase (LPL) and mediates its transport from the basolateral to the lumenal face of endothelial cells (6, 8). VLDL R knockout mice are characterized by reduced LPL activity, reduced serum triglyceride clearance, and a resistance to developing obesity (7, 9, 10). VLDL R influences breast cancer cell motility by mediating the uptake of uPAR-PAI1 complexes (6, 11). Lipoprotein accumulation via macrophage VLDL R is instrumental in promoting the formation of atherosclerotic plaques (12). In the nervous system, VLDL R and ApoE R2 interactions with Reelin are critical for neuronal migration and positioning in the developing brain (13). VLDL R also functions in adult hippocampal synapse maturation, synaptic plasticity, and memory formation (14, 15). The ECD of mouse VLDL R shares 95% aa sequence identity with human and rat VLDL R. Within shared regions, mouse VLDL R shares 55% and 53% aa sequence identity with ApoE R2 and LDL R, respectively.
- Qiu, S. et al. (2006) Neurobiol. Learn. Mem. 85:16.
- May, P. et al. (2005) Cell. Mol. Life Sci. 62:2325.
- Gafvels, M.E. et al. (1994) Endocrinology 135:387.
- Oka, K. et al. (1994) Eur. J. Biochem. 224:975.
- Wyne, K.L. et al. (1996) Arterioscler. Thromb. Vasc. Biol. 16:407.
- Argraves, K.M. et al. (1995) J. Biol. Chem. 270:26550.
- Goudriaan, J.R. et al. (2001) Arterioscler. Thromb. Vasc. Biol. 21:1488.
- Obunike, J.C. et al. (2001) J. Biol. Chem. 276:8934.
- Yagyu, H. et al. (2002) J. Biol. Chem. 277:10037.
- Goudriaan, J.R. et al. (2004) J. Lipid Res. 45:1475.
- Webb, D.J. et al. (1999) J. Biol. Chem. 274:7412.
- van Eck, M. et al. (2005) Atherosclerosis 183:230.
- Jossin, Y. et al. (2004) J. Neurosci. 24:514.
- Niu, S. et al. (2004) Neuron 41:71.
- Weeber, E.J. et al. (2002) J. Biol. Chem. 277:39944.
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