Mouse TWEAK/TNFSF12 Antibody Summary
Arg105-His249
Accession # O54907
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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TWEAK/TNFSF12 Inhibition of Cell Proliferation and Neutralization by Mouse TWEAK/TNFSF12 Antibody. Recombinant Mouse TWEAK/TNFSF12 (Catalog # 1237-TW) inhibits proliferation in the the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line). Activity elicited by Recombinant Mouse TWEAK/TNFSF12 (2 µg/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse TWEAK/ TNFSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1237). The ND50 is typically 4-16 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TWEAK/TNFSF12
TNF-related weak inducer of apoptosis (TWEAK) is a type II transmembrane protein belonging to the TNF superfamily and has been designated TNFSF12. Mouse TWEAK is a 249 amino acid (aa) protein with an N-terminal 21 aa cytoplasmic domain, a 21 aa transmembrane region and a 204 aa C-terminal extracellular domain (1). The primary structures of the extracellular domains of human and mouse TWEAK are 88% identical. A soluble form of TWEAK is generated from the membrane-associated molecules by proteolytic cleavage suggesting that TWEAK may have long-range effects. TWEAK is expressed widely in many tissues and cells (1). Although TWEAK has been proposed as a ligand that signals through the death domain receptor 3 (DR3) (2), a TNF receptor superfamily member currently designated TNFRSF25, subsequent studies did not demonstrate binding of TWEAK to cell lines that express DR3 (3). In cells that lack DR3, TWEAK has been shown to bind TWEAK receptor (TWEAK R), a novel TNF receptor superfamily member designated TNFRSF12A (4‑7). TWEAK R, also known as fibroblast growth factor-inducible 14 (Fn14), is a growth factor-inducible immediate-early response gene that is expressed in fibroblasts, hepatocellular carcinomas and endothelial cells. TWEAK-TWEAK R interaction has been shown to promote NF-kappa B activation and mediate multiple cell death pathways. On endothelial cells, TWEAK R plays a role in endothelial cell growth and migration. This effect of TWEAK is not due to upregulation of VEGF (8).
- Chicheportiche, Y. et al. (1997) J. Biol Chem. 272:32401.
- Marsters, S. et al. (1998) Current Biol. 8:525.
- Kaptein, A. et al. (2000) FEBS Letters, 485:135.
- Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.
- Nakayama, M. et al. (2002) J. Immunol. 168:734.
- Feng, S.L. et al. (2000) Am. J. Pathol. 156:1253.
- Wiley, S.R. et al. (2001) Immunity 15:837.
- Lynch, C. et al. (1998) J. Biol. Chem. 274:8455.
Product Datasheets
Citation for Mouse TWEAK/TNFSF12 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Sensory Experience Engages Microglia to Shape Neural Connectivity through a Non-Phagocytic Mechanism
Authors: Lucas Cheadle, Samuel A. Rivera, Jasper S. Phelps, Katelin A. Ennis, Beth Stevens, Linda C. Burkly et al.
Neuron
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