Mouse TSG Antibody Summary
Cys25-Phe222
Accession # Q9EP52
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
TSG in Mouse Embryo. TSG was detected in immersion fixed frozen sections of mouse embryo using 15 µg/mL Goat Anti-Mouse TSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF756) overnight at 4 °C. Tissue was stained (red) and counter-stained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
View Larger
TSG in Mouse Embryo. TSG was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse TSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF756) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TSG
Twisted Gastrulation (TSG) is a secreted, cysteine-rich protein that plays a role in dorsal/ventral patterning by regulating BMP signaling in Drosophila and Xenopus. TSG was originally identified in Drosophila melanogaster and shown to be required for the differentiation of the dorsal amnioserosa cells. Vertebrate TSGs were subsequently cloned in mouse, human, zebrafish and frog. Mouse TSG encodes a 222 amino acid (aa) residue precursor protein with a 24 aa residue putative signal peptide that is cleaved to generate the 198 aa residue mature protein. Studies of expression and function of Twisted Gastrulation have been performed in Drosophila and Xenopus. Xenopus TSG is expressed in the ventral regions of the embryo during gastrulation, mimicking the BMP-4 expression pattern. dTSG is expressed in dorsal cells of the blastoderm embryo, where there are also high levels of activity of Dpp and Screw. In vivo, TSG acts as an agonist for BMP signaling by modulating the inhibitory actions of the BMP antagonist, Chordin/Sog and the cleavage properties of the metalloprotease, xolloid/tolloid. The N-terminal domain of TSG can bind BMP protein directly in vitro and shows BMP antagonist activity.
- Mason, et al. (1994) Genes Dev. 8:1489.
- Oelgeschlager, et al. (2000) Nature 405:757.
- Yu, et al. (2000) Development 127:2143.
- Dale (2000) Current Biology 10:R671.
Product Datasheets
Citation for Mouse TSG Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
High levels of GDF15 in thalassemia suppress expression of the iron regulatory protein hepcidin.
Authors: Tanno T, Bhanu NV, Oneal PA, Goh SH, Staker P, Lee YT, Moroney JW, Reed CH, Luban NL, Wang RH, Eling TE, Childs R, Ganz T, Leitman SF, Fucharoen S, Miller JL
Nat. Med., 2007-08-26;13(9):1096-101.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Mouse TSG Antibody
There are currently no reviews for this product. Be the first to review Mouse TSG Antibody and earn rewards!
Have you used Mouse TSG Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
