Mouse TREML2/TLT-2 Antibody

Catalog # Availability Size / Price Qty
AF6354
AF6354-SP
Detection of Mouse TREML2/TLT‑2 by Western Blot.
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Mouse TREML2/TLT-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TREML2/TLT-2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) TREML1, rmTREML4, rmTREM1, rmTREM2b, rmTREM3, rmTREM4, and recombinant human TREM2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse TREML2/TLT-2
His25-Ala270
Accession # Q2LA85
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse TREML2/TLT-2 antibody by Western Blot. View Larger

Detection of Mouse TREML2/TLT‑2 by Western Blot. Western blot shows lysates of mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Mouse TREML2/TLT-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6354) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A band was detected for TREML2/TLT-2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Flow Cytometry Detection of TREML2/TLT-2 antibody in M1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of TREML2/TLT-2 in M1 Mouse Cell Line by Flow Cytometry. M1 mouse myeloid leukemia cell line was stained with Mouse TREML2/TLT-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6354, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREML2/TLT-2

Mouse Trem-like transcript 2 protein (TLT-2; also TREML2) is a 34 kDa (predicted for unglycosylated) type I transmembrane cell surface receptor and member of the Trem family of receptor proteins. Mouse TLT-2 is synthesized as a 329 amino acid (aa) precursor that contains a 24 aa signal sequence, a 246 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 38 aa cytoplasmic tail. Within the ECD are an Ig-like V-type domain (aa 25-126) and one potential site for N-linked glycosylation. Mature mouse TLT-2 shares 49% aa sequence identity with mature human TLT-2. Cell surface expression of TLT-2 is seen for most of the B and myeloid cell lines. TLT-2 is also expressed constitutively on CD8+ T cells.

Long Name
Triggering Receptor Expressed on Myeloid Cells-like 2
Entrez Gene IDs
79865 (Human); 328833 (Mouse)
Alternate Names
C6orf76; dJ238O23.1; FLJ13693; MGC149715; MGC149716; TLT2; TLT-2; TREML2; trem-like transcript 2 protein; triggering receptor expressed on myeloid cells-like 2; triggering receptor expressed on myeloid cells-like protein 2; UNQ6268/PRO20473

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