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Mouse TLR4 Antibody

Catalog #: MAB2759
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2759
MAB2759-SP
TLR4 in RAW 264.7 Mouse Monocyte/Macrophage Cell Line.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TLR4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TLR4 in direct ELISAs. In direct ELISAs, does not cross-react with recombinant mouse TLR1, 2, 3, 6, or recombinant human TLR4.
Source
Monoclonal Rat IgG2A Clone # 267518
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TLR4
Asn26-Lys629
Accession # Q9QUK6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
RAW 264.7 mouse monocyte/macrophage cell line treated with LPS, fixed with paraformaldehyde and permeabilized with saponin
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry TLR4 antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Monocyte/Macrophage Cell Line by Immunocytochemistry (ICC). View Larger

TLR4 in RAW 264.7 Mouse Monocyte/Macrophage Cell Line. TLR4 was detected in immersion fixed RAW 264.7 mouse monocyte/ macrophage cell line using Rat Anti-Mouse TLR4 Monoclonal Antibody (Catalog # MAB2759) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR4

TLR4 belongs to the IL-1 receptor/Toll-like receptor superfamily. It is expressed in placenta and peripheral blood leukocytes. TLR4 is the pattern recognition receptor for LPS.

Long Name
Toll-like Receptor 4
Entrez Gene IDs
7099 (Human); 21898 (Mouse); 29260 (Rat)
Alternate Names
ARMD10; CD_antigen: CD284; CD284 antigen; CD284; EC 3.2.2.6; EC:3.2.2.6; homolog of Drosophila toll; hToll; TLR4; TLR-4; toll like receptor 4 protein; TOLL; toll-like receptor 4

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Citations for Mouse TLR4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Anagliptin prevents lipopolysaccharide (LPS)- induced inflammation and activation of macrophages
    Authors: F Yu, W Tian, J Dong
    International immunopharmacology, 2022-01-16;104(0):108514.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Deletion of Galectin-3 attenuates acute pancreatitis in mice by affecting activation of innate inflammatory cells
    Authors: B Stojanovic, I Jovanovic, BS Stojanovic, MD Stojanovic, N Gajovic, G Radosavlje, J Pantic, N Arsenijevi, ML Lukic
    Eur. J. Immunol., 2019-04-02;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Absence of toll-like receptor 4 (TLR4) extends survival in the hSOD1 G93A mouse model of amyotrophic lateral sclerosis.
    Authors: Lee J, Lee J, Phipps S, Noakes P, Woodruff T
    J Neuroinflammation, 2015-05-13;12(0):90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  4. Vaginal epithelial cell-derived S100 alarmins induced by Candida albicans via pattern recognition receptor interactions are sufficient but not necessary for the acute neutrophil response during experimental vaginal candidiasis.
    Authors: Yano J, Palmer G, Eberle K, Peters B, Vogl T, McKenzie A, Fidel P
    2013-12-09;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  5. Adjuvant effect of killed Propionibacterium acnes on mouse peritoneal B-1 lymphocytes and their early phagocyte differentiation.
    Authors: Mussalem JS, Squaiella-Baptistao CC, Teixeira D, Yendo TM, Thies FG, Popi AF, Mariano M, Longo-Maugeri I
    PLoS ONE, 2012-03-20;7(3):e33955.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. VISA Is Required for B Cell Expression of TLR7
    Authors: Liang-Guo Xu, Lei Jin, Bi-Cheng Zhang, Linda J. Akerlund, Hong-Bing Shu, John C. Cambier
    The Journal of Immunology
  7. Toll-like receptor 4 regulates early endothelial activation during ischemic acute kidney injury.
    Authors: Chen J, John R, Richardson JA, Shelton JM, Zhou XJ, Wang Y, Wu QQ, Hartono JR, Winterberg PD, Lu CY
    Kidney Int., 2010-10-06;79(3):288-99.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Hemorrhagic shock augments lung endothelial cell activation: role of temporal alterations of TLR4 and TLR2.
    Authors: Li Y, Xiang M, Yuan Y, Xiao G, Zhang J, Jiang Y, Vodovotz Y, Billiar TR, Wilson MA, Fan J
    Am. J. Physiol. Regul. Integr. Comp. Physiol., 2009-10-14;297(6):R1670-80.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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