Mouse TLR1 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1475P
Detection of TLR1 in A20 Mouse Cell Line by Flow Cytometry.
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Mouse TLR1 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TLR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse (rm) TLR6 is observed, 5% cross-reactivity with recombinant human (rh) TLR1 and rmTLR2 is observed, and less than 2% cross-reactivity with rhTLR3 and rhTLR4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TLR1
Ser25-Asp581
Accession # Q9EPQ1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TLR1 antibody in A20 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of TLR1 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse TLR1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1475P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TLR1

The Toll-like family of molecules are type I transmembrane proteins that serve as pattern recognition receptors for microbial pathogens. There are at least twelve mouse and ten human TLRs that activate the innate immune system following exposure to a variety of microbial species (1, 2). TLRs contain a large number of leucine-rich repeats (LRRs) and a cytoplasmic tail with one Toll/IL-1 receptor (TIR) domain. Mature mouse TLR1 consists of a 557 amino acid (aa) extracellular domain (ECD) with 20 LRRs, a 21 aa transmembrane segment, and a 192 aa cytoplasmic domain (3, 4). Within the ECD, mouse TLR1 shares 60% aa sequence identity with mouse TLR6 and 19%‑27% aa sequence identitity with mouse TLR2, -3, -4, -5, -7, -8, -9, -11, -12, and -13. It shares 73% and 86% aa sequence identity with human and rat TLR1, respectively. TLR1 is expressed on the surface of macrophages, dendritic cells, and tonsillar epithelial cells in ligand-independent association with TLR2 (5‑8). TLR2 additionally associates with TLR6 to form a functional complex with specificity for distinct but related microbial ligands (9‑11). TLR1 and TLR2 cooperate in the recognition of bacterial and protozoal triacylated lipopeptides and glycosylphosphatidylinositols (6, 10‑12). Ligand binding induces TLR1 localization to lipid rafts followed by receptor internalization and activation of NF kappa B (7, 11, 13).

References
  1. Miyake, K. (2007) Semin. Immunol. 19:3.
  2. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  3. SwissProt # Q9EPQ1.
  4. Matsushima, N. et al. (2007) BMC Genomics 8:124.
  5. Ochoa, M.-T. et al. (2003) Immunology 108:10.
  6. Takeuchi, O. et al. (2002) J. Immunol. 169:10.
  7. Triantafilou, M. et al. (2006) J. Biol. Chem. 281:31002.
  8. Sandor, F. et al. (2003) J. Cell Biol. 162:1099.
  9. Nakao, Y. et al. (2005) J. Immunol. 174:1566.
  10. Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. 97:13766.
  11. Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
  12. Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
  13. Nishiya, T. and A.L. DeFranco (2004) J. Biol. Chem. 279:19008.
 
Long Name
Toll-like Receptor 1
Entrez Gene IDs
7096 (Human); 21897 (Mouse); 305354 (Rat)
Alternate Names
CD281 antigen; CD281; DKFZp547I0610; DKFZp564I0682; KIAA0012MGC126311; MGC104956; MGC126312; rsc786; TIL; TIL. LPRS5; TLR1; Toll/interleukin-1 receptor-like protein; toll-like receptor 1

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