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Mouse TGF-beta  RI/ALK-5 Antibody

Catalog #: MAB587
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB587
MAB587-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TGF-beta  RI/ALK-5 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TGF‑ beta  RI/ALK-5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 20‑25% cross-reactivity with recombinant human (rh) TGF-beta RI and no cross-reactivity with recombinant rat MIS RII, rhTGF-beta RII, rhTGF‑ beta  RIIb, or rhTGF‑ beta  RIII.
Source
Monoclonal Rat IgG2A Clone # 141229
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF‑ beta  RI/ALK-5
Ala21-Glu121
Accession # BAA05023
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse TGF-beta  RI/ALK‑5 Fc Chimera (Catalog # 587-RI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RI/ALK-5

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor, originally known as ALK-5 (Activin receptor‑like kinase) is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

References
  1. Miyazono, K. et al. (1994) Adv. In Immunol. 55:181.
  2. Massagùe, J. (1998) Ann. Rev. Biochem. 67:753.
Long Name
Transforming Growth Factor beta Receptor I
Entrez Gene IDs
7046 (Human); 21812 (Mouse); 29591 (Rat)
Alternate Names
AAT5; activin A receptor type II-like kinase, 53kD; activin A receptor type II-like kinase, 53kDa; Activin receptor-like kinase 5; ACVRLK4; ALK-5; ALK-5ALK5; EC 2.7.11; EC 2.7.11.30; LDS1A; LDS2A; Serine/threonine-protein kinase receptor R4; SKR4; tbetaR-I; TGFB1R1; TGF-beta receptor type I; TGF-beta receptor type-1; TGF-beta RI; TGF-beta type I receptor; TGFbetaRI; TGFBR1; TGF-bRI; TGFR-1; transforming growth factor beta receptor I; transforming growth factor, beta receptor 1; transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD); Transforming growth factor-beta receptor type I

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Citations for Mouse TGF-beta  RI/ALK-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. TDAG51 induces renal interstitial fibrosis through modulation of TGF-beta receptor 1 in chronic kidney disease
    Authors: RE Carlisle, Z Mohammed-A, C Lu, T Yousof, V Tat, S Nademi, ME MacDonald, RC Austin, JG Dickhout
    Cell Death & Disease, 2021-10-08;12(10):921.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Modulation of TGF-beta signaling by endoglin in murine hemangioblast development and primitive hematopoiesis.
    Authors: Zhang L, Magli A, Catanese J, Xu Z, Kyba M, Perlingeiro RC
    Blood, 2011-05-20;118(1):88-97.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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