Mouse Sulfatase Modifying Factor 2/SUMF2 Antibody

Catalog # Availability Size / Price Qty
MAB3454
MAB3454-SP
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Mouse Sulfatase Modifying Factor 2/SUMF2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Sulfatase Modifying Factor 2/SUMF2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human SUMF2 or recombinant mouse SUMF1 is observed.
Source
Monoclonal Rat IgG2A Clone # 382407
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Sulfatase Modifying Factor 2/SUMF2
Gln34-Leu308
Accession # Q8BPG6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Sulfatase Modifying Factor 2/SUMF2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sulfatase Modifying Factor 2/SUMF2

Sulfatase Modifying Factor 2 (SUMF2) is structurally similar to SUMF1, which activates sulfatases by converting their active site residue cysteine to formylglycine. Functionally, SUMF2 does not activate sulfatases, but rather competes with SUMF1 for the same substrates. The amino acid sequence of mouse SUMF2 is 94%, 83%, 82%, 81% and 72% identical to that of rat, human, canine, chimpanzee and chicken.

Entrez Gene IDs
25870 (Human)
Alternate Names
C-alpha-formyglycine-generating enzyme 2; C-alpha-formylglycine-generating enzyme 2; DKFZp566I1024; DKFZp686I1024; DKFZp686L17160; DKFZp781L1035; FGE2; MGC99485; paralog of the formylglycine-generating enzyme; pFGE; sulfatase modifying factor 2; sulfatase-modifying factor 2; SUMF2

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