Mouse SPARC Biotinylated Antibody

Catalog #: BAF942
1 Citation Datasheet / COA / SDS

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BAF942

All SPARC Antibodies

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Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse SPARC Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse SPARC/Osteonectin in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human SPARC is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SPARC/Osteonectin
Ala18-Ile302
Accession # P07214

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse SPARC/Osteonectin (Catalog # 942-SP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SPARC

SPARC, an acronym for “secreted protein, acidic and rich in cysteine”, is also known as osteonectin or BM-40 (1‑5). It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 302 amino acid (aa), 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs (1‑5). Crystal structure shows that residues implicated in cell binding, inhibition of cell spreading and disassembly of focal adhesions cluster on one face of SPARC, while a collagen binding epitope and an N-glycosylation site are opposite this face (6). SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling (3, 7). SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation (3‑5). For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF (3‑5). SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis (5, 8). SPARC is potentially cleaved by metalloproteinases, producing an angiogenic peptide that includes the copper-binding sequence KGHK (7). Paradoxically, SPARC is highly expressed in many tumor types, yet expression mainly decreases the likelihood of metastasis and confers sensitivity to chemotherapy and radiation (4, 9, 10). Stabilin-1, which is expressed on alternately activated macrophages, is the first SPARC receptor to be identified. It binds the SPARC EC domain and mediates endocytosis for degradation (11). Mature mouse SPARC shows 97%, 92%, 92%, 92% and 83% aa identity with rat, human, dog, cow and chick SPARC, respectively.

References

Lankat-Buttgereit, B. et al. (1988) FEBS Lett. 236:352.
McVey, J.H. et al. (1988) J. Biol. Chem. 263:11111.
Sage, H. et al. (1989) J. Cell Biol. 109:341.
Framson, P.E. and E.H. Sage (2004) J. Cell. Biochem. 92:679.
Alford, A.I. and K.D. Hankenson (2006) Bone 38:749.
Hohenester, E. et al. (1997) EMBO J. 16:3778.
Sage, E.H. et al. (2003) J. Biol. Chem. 278:37849.
Delany, A.M. et al. (2003) Endocrinology 144:2588.
Koblinski, J.E. et al. (2005) Cancer Res. 65:7370.
Tai, I.T. et al. (2005) J. Clin. Invest. 115:1492.
Kzhyshkowska, J. et al. (2006) J. Immunol. 176:5825.

Long Name

Secreted Protein Acidic and Rich in Cysteine

Entrez Gene IDs

6678 (Human); 20692 (Mouse)

Alternate Names

Basement-membrane protein 40; BM-40; ONcysteine-rich protein; Osteonectin; Secreted protein acidic and rich in cysteine; secreted protein, acidic, cysteine-rich (osteonectin); SPARC

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Mouse SPARC Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Absence of SPARC results in increased cardiac rupture and dysfunction after acute myocardial infarction.
Authors: Schellings MW, Vanhoutte D, Swinnen M, Cleutjens JP, Debets J, van Leeuwen RE, d'Hooge J, Van de Werf F, Carmeliet P, Pinto YM, Sage EH, Heymans S
J. Exp. Med., 2008-12-22;206(1):113-23.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr