Mouse Sonic Hedgehog/Shh N-Terminus Biotinylated Antibody

Catalog #: BAM4642
1 Citation Datasheet / COA / SDS

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BAM4642

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Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Sonic Hedgehog/Shh N-Terminus Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Sonic Hedgehog (Shh) N-terminal peptide in ELISAs. In ELISAs, this antibody shows approximately 10% cross-reactivity with recombinant human (rh) Shh N-terminal peptide but no cross-reactivity with rmDhh N-terminal (aa 23‑198) or C‑terminal (aa 199‑396) peptides, rmShh C-terminal peptide (aa 199-437), or rmHIP.

Source

Monoclonal Rat IgG_2A Clone # 171028

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Sonic Hedgehog N-Terminus
Cys25-Gly198 (Lys122Arg)
Accession # Q62226

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Mouse Sonic Hedgehog Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Mouse Sonic Hedgehog/Shh N-Terminus Antibody (Catalog # MAB4641)

Standard: Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus Protein (Catalog # 461-SH)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sonic Hedgehog/Shh

Shh is a hedgehog protein that is instrumental in patterning the early embryo. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened.

Entrez Gene IDs

6469 (Human); 20423 (Mouse)

Alternate Names

HHG1; HHG-1; HLP3; HPE3; MCOPCB5; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; ShhNC; SMMCI; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Mouse Sonic Hedgehog/Shh N-Terminus Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Sonic hedgehog and other soluble factors from differentiating embryoid bodies inhibit pancreas development.
Authors: Mfopou JK, De Groote V, Xu X, Heimberg H, Bouwens L
Stem Cells, 2007-02-01;25(5):1156-65.
Species: Mouse
Sample Types: Whole Cells
Applications: ELISA Development