Mouse Sonic Hedgehog/Shh N-Terminus Antibody Summary
(N‑terminus; aa 23‑198) is observed and no cross-reactivity with rmIhh (N-terminus; aa 66‑240), rmDhh (C‑terminus; aa 199‑396), or rmShh (C‑terminus; aa 199-437) is observed.
Cys25-Gly198 (Lys122Arg)
Accession # Q62226
Applications
Mouse Sonic Hedgehog Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Alkaline Phosphatase Production Induced by Sonic Hedgehog and Neutralization by Mouse Sonic Hedgehog Antibody. Recombinant Mouse Sonic Hedgehog N-Terminus (Catalog # 461-SH or 464-SH) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Mouse Sonic Hedgehog N-Terminus (5 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Sonic Hedgehog N-Terminus Monoclonal Antibody (Catalog # MAB4641). The ND50 is typically 5-15 µg/mL.
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Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot. Western blot shows lysates of human brain tissue, human stomach tissue, mouse brain tissue, and mouse stomach tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB4641) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Sonic Hedgehog/Shh in Mouse Embryo. Sonic Hedgehog/Shh was detected in immersion fixed frozen sections of mouse embryo (11 d.p.c.) using Rat Anti-Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB4641) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sonic Hedgehog/Shh
Sonic Hedgehog (Shh) is a hedgehog protein that is instrumental in patterning the early embryo. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened.
Product Datasheets
Citations for Mouse Sonic Hedgehog/Shh N-Terminus Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Sonic Hedgehog Promotes Tumor Cell Survival by Inhibiting CDON Pro-Apoptotic Activity
Authors: Céline Delloye-Bourgeois, Benjamin Gibert, Nicolas Rama, Jean-Guy Delcros, Nicolas Gadot, Jean-Yves Scoazec et al.
PLoS Biology
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Sonic hedgehog secreted by neurons regulates angiopoietin expression in neighboring fibroblasts
Authors: WOO JEAN KIM, SAE-WON LEE, KYU-WON KIM
International Journal of Molecular Medicine
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Oncogenic KRAS Regulates Tumor Cell Signaling via Stromal Reciprocation
Authors: Christopher J. Tape, Stephanie Ling, Maria Dimitriadi, Kelly M. McMahon, Jonathan D. Worboys, Hui Sun Leong et al.
Cell
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The acid-secreting parietal cell as an endocrine source of Sonic Hedgehog during gastric repair.
Authors: Engevik A, Feng R, Yang L, Zavros Y
Endocrinology, 2013-10-03;154(12):4627-39.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Sonic hedgehog and other soluble factors from differentiating embryoid bodies inhibit pancreas development.
Authors: Mfopou JK, De Groote V, Xu X, Heimberg H, Bouwens L
Stem Cells, 2007-02-01;25(5):1156-65.
Species: Mouse
Sample Types: Cell Culture Supernates, Whole Cells
Applications: ELISA Development, Neutralization -
Plasma Shh Levels Reduced in Pancreatic Cancer Patients
Authors: Mohamad El-Zaatari, Stephanie Daignault, Art Tessier, Gail Kelsey, Lisa A. Travnikar, Esperanza F. Cantu et al.
Pancreas
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Hedgehog Promotes Neovascularization in Pancreatic Cancers by Regulating Ang-1 and IGF-1 Expression in Bone-Marrow Derived Pro-Angiogenic Cells
Authors: Kazumasa Nakamura, Junpei Sasajima, Yusuke Mizukami, Yoshiaki Sugiyama, Madoka Yamazaki, Rie Fujii et al.
PLoS ONE
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