Mouse Serpin E2/PN1 Biotinylated Antibody

Catalog #: BAF2175 Datasheet / COA / SDS

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BAF2175

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Mouse Serpin E2/PN1 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Serpin E2/PN1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Fas, recombinant mouse Fas, and recombinant feline Fas (under non-reducing conditions) is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Serpin E2/PN1
Ser20-Pro397
Accession # Q07235

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Serpin E2/PN1 (Catalog # 2175-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin E2/PN1

Serpin E2, also known as protease nexin I or glial-derived nexin (GDN), is a member of the Serpin superfamily of the serine protease inhibitors (1). Serpin E2 is a potent inhibitor of thrombin, plasmin and plasminogen activators (2). It is differentially expressed during neuronal differentiation and is able to transform human embryonic kidney cells into neuron-like cells (3). Its over‑expression in mice leads to progressive neuronal and motor dysfunction in these animals (4). It is also over‑expressed in the majority of pancreatic carcinoma as well as gastric and colorectal cancer samples whereas it is weakly expressed in all normal pancreas and chronic pancreatitis tissue samples (5). It plays an important role in controlling male fertility because its knockout male mice show a marked impairment in fertility from the onset of sexual maturity and its abnormal expression is found in the semen of men with seminal dysfunction (6).

References

Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
Rossignol, P. et al. (2004) J. Biol. Chem. 279:10346.
Lin, H.J. et al. (2005) Int. J. Dev. Neurosci. 23:9.
Meins, M. et al. (2001) J. Neurosci. 21:8830.
Buchholz, M. et al. (2003) Cancer Res. 63:4945.
Murer, V. et al. (2001) Proc. Natl. Acad. Sci. USA 98:3029.

Entrez Gene IDs

5270 (Human); 20720 (Mouse)

Alternate Names

GDN; GdNPF; GDNserine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 2; glia-derived nexin; glial-derived neurite promoting factor; Peptidase inhibitor 7; PI-7; PI7DKFZp686A13110; PN-1; PN1nexin; PNI; Protease nexin 1; Protease nexin I; Proteinase Nexin 1; Serpin E2; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 2