Home / RGM-C/Hemojuvelin / Mouse RGM-C Antibody

Mouse RGM-C Antibody

Catalog #: AF3634
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3634
AF3634-SP
Product Details
Citations (6)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse RGM-C Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse RGM-C in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human RGM‑C is observed and less than 5% cross-reactivity with recombinant mouse (rm) RGM-A and rmRGM-B is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse RGM-C isoform 1 (R&D Systems, Catalog # 3634-RG)
Gln33-Asp393 (Ile379Val)
Accession # Q7TQ32
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse RGM-C (Catalog # 3634-RG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RGM-C/Hemojuvelin

RGM-C, also known as hemojuvelin, is a member of the repulsive guidance molecule (RGM) family of GPI-linked neuronal and muscle membrane glycoproteins (1). RGM‑C is expressed in striated muscle and periportal hepatocytes (2-4). The protein undergoes partial cleavage intracellularly, resulting in a disulfide-linked dimer of the 14 kDa N-terminal and 33 kDa C-terminal portions (3, 5, 6). The N-terminal fragment contains an RGD motif, while the C-terminal fragment carries the GPI attachment site (3, 6). An alternatively spliced isoform lacks the N-terminal fragment. Full length RGM-C can also be released from the cell and circulates in the blood (5, 7). RGM-C is disrupted in type 2A juvenile hemochromatosis, a hereditary iron homeostasis disorder characterized by excessive iron accumulation (4). Loss of RGM-C function results in decreased expression of the iron regulatory hormone hepicidin and increased iron deposition in liver, pancreas, and heart (4, 8). Membrane associated RGM-C upregulates hepicidin while soluble RGM-C downregulates hepicidin expression (7). This appears to be an iron-responsive regulatory system, as high blood iron levels reduce the amount of soluble RGM-C produced (7). RGM-C, similar to RGM-A, associates with neogenin (6). Disease-related point mutations can prevent internal RGM-C cleavage or its ability to interact with neogenin (5, 6). Experimental inflammatory conditions result in decreased RGM-C expression and increased hepicidin expression, although the two effects occur independently (4, 9). RGM-C also functions as a BMP co-receptor and enhances BMP-2 and BMP-4 signaling (10). In this context, RGM-C enhances the BMP-2 upregulation of hepatic hepicidin (10). Mature mouse RGM-C shares 89% and 97% amino acid (aa) sequence identity with human and rat RGM-C, respectively. It shares 51% and 44% aa sequence identity with mouse RGM-A and RGM-B, respectively.

References
  1. Schmidtmer, J. and D. Engelkamp (2004) Gene Exp. Patterns 4:105.
  2. Oldekamp, J. et al. (2004) Gene Exp. Patterns 4:283.
  3. Niederkofler, V. et al. (2004) J. Neurosci. 24:808.
  4. Niederkofler, V. et al. (2005) J. Clin. Invest. 115:2180.
  5. Kuninger, D. et al. (2006) J. Cell Sci. 119:3273.
  6. Zhang, A.S. et al. (2005) J. Biol. Chem. 280:33885.
  7. Lin, L. et al. (2005) Blood 106:2884.
  8. Huang, F.W. et al. (2005) J. Clin. Invest. 115:2187.
  9. Krijt, J. et al. (2004) Blood 104:4308.
  10. Babitt, J.L. et al. (2006) Nat. Genet. 38:531.
Long Name
Repulsive Guidance Molecule C
Entrez Gene IDs
148738 (Human); 69585 (Mouse); 310681 (Rat)
Alternate Names
DL-M; haemojuvelin; hemochromatosis type 2 (juvenile); Hemojuvelin; HFE2; HFE2AMGC23953; HJV; HJVHemochromatosis type 2 protein; JH; repulsive guidance molecule c; RGMC; RGM-C; RGMCRGM domain family member C

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Citations for Mouse RGM-C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. HFE inhibits type I IFNs signaling by targeting the SQSTM1-mediated MAVS autophagic degradation
    Authors: J Liu, X Wu, H Wang, J Wei, Q Wu, X Wang, Y Yan, J Cui, J Min, F Wang, J Zhou
    Autophagy, 2020-08-18;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Effect of erythropoietin administration on proteins participating in iron homeostasis in Tmprss6-mutated mask mice
    Authors: J Frýdlová, Z Rychtar?ík, I Gurieva, M Vokurka, J Truksa, J Krijt
    PLoS ONE, 2017-10-26;12(10):e0186844.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Erythropoietin administration increases splenic erythroferrone protein content and liver TMPRSS6 protein content in rats
    Authors: I Gurieva, J Frýdlová, Z Rychtar?ík, M Vokurka, J Truksa, J Krijt
    Blood Cells Mol. Dis, 2017-02-28;64(0):1-7.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Effect of Erythropoietin, Iron Deficiency and Iron Overload on Liver Matriptase-2 (TMPRSS6) Protein Content in Mice and Rats.
    Authors: Frydlova J, Prikryl P, Truksa J, Falke L, Du X, Gurieva I, Vokurka M, Krijt J
    PLoS ONE, 2016-02-04;11(2):e0148540.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. A novel validated enzyme-linked immunosorbent assay to quantify soluble hemojuvelin in mouse serum
    Authors: Wenjie Chen, Chia Chi Sun, Shanzhuo Chen, Delphine Meynard, Jodie L Babitt, Herbert Y Lin
    Haematologica
  6. Liver hemojuvelin protein levels in mice deficient in matriptase-2 (Tmprss6).
    Authors: Krijt J, Fujikura Y, Ramsay AJ, Velasco G, Necas E
    Blood Cells Mol. Dis., 2011-05-25;47(2):133-7.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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