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Mouse Reg3D Antibody

Catalog #: MAB5678
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5678
MAB5678-SP
Detection of Reg3D in Mouse Pancreas.
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Citations (1)
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Mouse Reg3D Antibody Summary

Species Reactivity
Mouse
Specificity
This antibody detects mouse Reg3D in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Reg1A, 1B, recombinant mouse (rm) Reg1, 2, 3A, 3B, 3G, 4, or recombinant rat Reg3 is observed.
Source
Monoclonal Rat IgG2A Clone # 518818
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Reg3D
Glu27-Gly175
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of mouse pancreas

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Detection of Reg3D in Mouse Pancreas. Reg3D was detected in immersion fixed paraffin-embedded sections of mouse pancreas using Rat Anti-Mouse Reg3D Monoclonal Antibody (Catalog # MAB5678) at 15 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005) or the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reg3D

Reg3D (regenerating islet-derived III delta), also called INGAPrP (islet neogenesis-associated protein-related protein) is an ~20 kDa member of the Reg family of secreted calcium-dependent C-type lectins. Reg proteins are involved in various biological functions, including acting as a survival/growth factors. Reg3D mRNA is expressed mainly in pancreatic islets and the digestive system. The mature 149 amino acid Reg3D shares ~50 - 70% amino acid sequence identity with other mammalian Reg3 proteins.

Long Name
Regenerating Islet-derived III delta
Entrez Gene IDs
30053 (Mouse)
Alternate Names
INGAPrP; islet neogenesis associated protein-related protein;Reg III delta;regenerating islet-derived 3 delta; Reg3D

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Citation for Mouse Reg3D Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Genetic and pharmacologic inhibition of ALDH1A3 as a treatment of beta-cell failure
    Authors: J Son, W Du, M Esposito, K Shariati, H Ding, Y Kang, D Accili
    Nature Communications, 2023-02-02;14(1):558.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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