Mouse/Rat Kynureninase Antibody

Catalog #: AF7389 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7389
AF7389-SP

Detection of Mouse and Rat Kynureninase by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat Kynureninase Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse and rat Kynureninase in Western blots. Detects recombinant mouse Kynureninase in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human Kynureninase is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Kynureninase
Gly267-Ser464
Accession # Q9CXF0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse and Rat Kynureninase by Western Blot. Western blot shows lysates of mouse liver tissue and rat liver tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Mouse Kynureninase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7389) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Kynureninase at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kynureninase

Kynu (kyureninase (a hydrolase that acts on dog [Greek: kyon] urine [Greek: ouron]) is a 52-55 kDa member of the kyureninase family of enzymes. It is a pyridoxal phosphate-dependent cytosolic hydrolase that occurs in a variety of cell types, including hepatocytes and macrophages. Kynu participates in the metabolism of tryptophan. Dietary Trp is either incorporated into protein, or metabolized into niacin, serotonin or NAD. Kynu catalyzes one of two steps that lead to the formation of a key downstream intermediate called 3-hydroxyanthranilic acid. Mouse Kynu is 464 amino acids (aa) in length. It contains one large catalytic site (aa 24-462), and typically functions as a noncovalent homodimer. There are at least two potential isoform variants. One contains a three aa substitution for aa 425-464, while another possesses a 22 aa substitution for aa 302-464. Over aa267-464 mouse Kynu shares 91% and 80% aa sequence identity with rat and human Kynu, respectively.

Entrez Gene IDs

8942 (Human); 70789 (Mouse); 116682 (Rat)

Alternate Names

EC 3.7.1.3; KYNU; kynureninase (L-kynurenine hydrolase); Kynureninase; L-kynurenine hydrolase