Home / Rae-1 / Mouse Rae-1 Pan Specific Antibody

Mouse Rae-1 Pan Specific Antibody

Catalog #: MAB17582
24 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB17582-SP
MAB17582-500
MAB17582-100
Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Citations (24)
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Mouse Rae-1 Pan Specific Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Rae‑1. It recognizes Rae-1 alpha, beta, δ, gamma and epsilon.
Source
Monoclonal Rat IgG2A Clone # 186107
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ
Leu29-Ser227
Accession # Q9JI58
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Rae-1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae-1 Pan Specific Monoclonal Antibody (Catalog # MAB17582, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rae-1

Rae-1 alpha, beta, gamma, δ and epsilon comprise a family of closely related (88-95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection.

Long Name
Retinoic Acid Early Transcript 1
Entrez Gene IDs
8480 (Human); 19368 (Mouse)
Alternate Names
dJ481F12.3;dJ800J21.1;Gle2;MIG14;Mnrp41;mRNA export factor RAE1;MRNP41;RAE1; Rae1; Rae-1

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Citations for Mouse Rae-1 Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Impaired autophagy promotes hair loss in the C3H/HeJ mouse model of alopecia areata
    Authors: R Gund, AM Christiano
    Autophagy, 2022-06-02;0(0):1-10.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Tumor hypoxia represses &gamma&delta T cell-mediated antitumor immunity against brain tumors
    Authors: JH Park, HJ Kim, CW Kim, HC Kim, Y Jung, HS Lee, Y Lee, YS Ju, JE Oh, SH Park, JH Lee, SK Lee, HK Lee
    Nature Immunology, 2021-02-11;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Gut microbiota alterations affect glioma growth and innate immune cells involved in tumor immunosurveillance in mice
    Authors: G D'Alessand, F Antonangel, F Marrocco, A Porzia, C Lauro, A Santoni, C Limatola
    Eur. J. Immunol., 2020-03-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Loss of NKG2D in murine NK cells leads to increased perforin production upon long-term stimulation with IL-2
    Authors: D Prinz, K Klein, J List, VM Knab, I Menzl, N Leidenfros, G Heller, B Poli?, EM Putz, A Witalisz-S, V Sexl, D Gotthardt
    Eur. J. Immunol., 2020-02-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Kbtbd2 inhibits the cytotoxic activity of immortalized NK cells through down-regulating mTOR signaling in a mouse hepatocellular carcinoma model
    Authors: K Dai, Y Huang, Z Chen, X Sun, L Yang, Y Jiang
    Eur. J. Immunol., 2018-01-29;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Chronic In Vivo Interaction of Dendritic Cells Expressing the Ligand Rae-1epsilon with NK Cells Impacts NKG2D Expression and Function.
    Authors: Morvan M, Champsaur M, Reizis B, Lanier L
    Immunohorizons, 2017-05-01;1(3):10-19.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. A Herpesviral induction of RAE-1 NKG2D ligand expression occurs through release of HDAC mediated repression
    Elife, 2016-11-22;5(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. NKG2D-NKG2D Ligand Interaction Inhibits the Outgrowth of Naturally Arising Low-Grade B Cell Lymphoma In Vivo
    J Immunol, 2016-05-02;196(11):4805-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. NKp46+ Innate Lymphoid Cells Dampen Vaginal CD8 T Cell Responses following Local Immunization with a Cholera Toxin-Based Vaccine.
    Authors: Luci C, Bekri S, Bihl F, Pini J, Bourdely P, Nouhen K, Malgogne A, Walzer T, Braud V, Anjuere F
    PLoS ONE, 2015-12-02;10(12):e0143224.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Overexpression of the transcription factor Sp1 activates the OAS-RNAse L-RIG-I pathway.
    Authors: Dupuis-Maurin V, Brinza L, Baguet J, Plantamura E, Schicklin S, Chambion S, Macari C, Tomkowiak M, Deniaud E, Leverrier Y, Marvel J, Michallet M
    PLoS ONE, 2015-03-04;10(3):e0118551.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity.
    Authors: Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C
    J Invest Dermatol, 2015-01-29;135(5):1311-9.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  12. Activating receptor NKG2D targets RAE-1-expressing allogeneic neural precursor cells in a viral model of multiple sclerosis.
    Authors: Weinger J, Plaisted W, Maciejewski S, Lanier L, Walsh C, Lane T
    Stem Cells, 2014-10-01;32(10):2690-701.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Ly49 family receptors are required for cancer immunosurveillance mediated by natural killer cells.
    Authors: Tu M, Mahmoud A, Wight A, Mottashed A, Belanger S, Rahim M, Abou-Samra E, Makrigiannis A
    Cancer Res, 2014-05-06;74(14):3684-94.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Engagement of NKG2D on bystander memory CD8 T cells promotes increased immunopathology following Leishmania major infection.
    Authors: Crosby E, Goldschmidt M, Wherry E, Scott P
    PLoS Pathog, 2014-02-27;10(2):e1003970.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. The E3 ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells.
    Authors: Paolino, Magdalen, Choidas, Axel, Wallner, Stephani, Pranjic, Blanka, Uribesalgo, Iris, Loeser, Stefanie, Jamieson, Amanda M, Langdon, Wallace, Ikeda, Fumiyo, Fededa, Juan Pab, Cronin, Shane J, Nitsch, Roberto, Schultz-Fademrecht, Carsten, Eickhoff, Jan, Menninger, Sascha, Unger, Anke, Torka, Robert, Gruber, Thomas, Hinterleitner, Reinhard, Baier, Gottfrie, Wolf, Dominik, Ullrich, Axel, Klebl, Bert M, Penninger, Josef M
    Nature, 2014-02-19;507(7493):508-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. RAE-1 ligands for the NKG2D receptor are regulated by E2F transcription factors, which control cell cycle entry.
    Authors: Jung, Heiyoun, Hsiung, Benjamin, Pestal, Kathleen, Procyk, Emily, Raulet, David H
    J Exp Med, 2012-11-19;209(13):2409-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Sustained CTL activation by murine pulmonary epithelial cells promotes the development of COPD-like disease.
    Authors: Borchers MT, Wesselkamper SC, Curull V, Ramirez-Sarmiento A, Sanchez-Font A, Garcia-Aymerich J, Coronell C, Lloreta J, Agusti AG, Gea J, Howington JA, Reed MF, Starnes SL, Harris NL, Vitucci M, Eppert BL, Motz GT, Fogel K, McGraw DW, Tichelaar JW, Orozco-Levi M
    J. Clin. Invest., 2009-02-09;119(3):636-49.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Intrinsic sensor of oncogenic transformation induces a signal for innate immunosurveillance.
    Authors: Unni AM, Bondar T, Medzhitov R
    Proc. Natl. Acad. Sci. U.S.A., 2008-01-25;105(5):1686-91.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Blockade of NKG2D on NKT cells prevents hepatitis and the acute immune response to hepatitis B virus.
    Authors: Vilarinho S, Ogasawara K, Nishimura S, Lanier LL, Baron JL
    Proc. Natl. Acad. Sci. U.S.A., 2007-11-08;104(46):18187-92.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Chimeric NKG2D receptor-bearing T cells as immunotherapy for ovarian cancer.
    Authors: Barber A, Zhang T, DeMars LR, Conejo-Garcia J, Roby KF, Sentman CL
    Cancer Res., 2007-05-15;67(10):5003-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. A role for activation-induced cytidine deaminase in the host response against a transforming retrovirus.
    Authors: Gourzi P, Leonova T, Papavasiliou FN
    Immunity, 2006-06-01;24(6):779-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. BCR/ABL promotes dendritic cell-mediated natural killer cell activation.
    Authors: Terme M, Borg C, Guilhot F, Masurier C, Flament C, Wagner EF, Caillat-Zucman S, Bernheim A, Turhan AG, Caignard A, Zitvogel L
    Cancer Res., 2005-07-15;65(14):6409-17.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Posttranslational regulation of the NKG2D ligand Mult1 in response to cell stress
    Authors: Timothy J. Nice, Laurent Coscoy, David H. Raulet
    Journal of Experimental Medicine
  24. Structural basis of mouse cytomegalovirus m152/gp40 interaction with RAE1 gamma reveals a paradigm for MHC/MHC interaction in immune evasion
    Authors: Rui Wang, Kannan Natarajan, Maria Jamela R. Revilleza, Lisa F. Boyd, Li Zhi, Huaying Zhao et al.
    Proceedings of the National Academy of Sciences

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