Mouse R-Spondin 4 Antibody

Catalog #: AF4106 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4106
AF4106-SP

All R-Spondin 4 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse R-Spondin 4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse R-Spondin 4 in direct ELISAs.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse R-Spondin 4
Tyr21-Pro197
Accession # Q8BJ73

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize R‑Spondin 4-induced activation of beta ‑Catenin response in the HEK293T human embryonic kidney cell line in a Topflash Luciferase assay. The Neutralization Dose (ND₅₀) is typically 5.0-20 µg/mL in the presence of 1 µg/mL Recombinant Mouse R‑Spondin 4 and 10 ng/mL Recombinant Mouse Wnt‑3a.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: R-Spondin 4

R-Spondin 4 (RSPO4, roof plate-specific spondin 4), also called cysteine-rich and single thrombospondin domain containing-4 (Cristin 4), is an ~33 kDa secreted heparin-binding protein that shares ~35% amino acid (aa) identity with three other R-Spondin family members (1-3). All are positive modulators of Wnt/ beta -catenin signaling, but R-Spondin 4 may be somewhat weaker than other R-Spondins (2). R‑Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK‑1‑mediated internalization (1, 4). Like other R‑Spondins, mouse R-Spondin 4 (234 aa) contains a signal sequence (aa 1-19), two adjacent cysteine-rich furin-like domains (aa 85-128) with one potential tyrosine phosphorylation site (aa 114), followed by a thrombospondin (TSP-1) motif (aa 137‑197) and a region rich in basic residues (aa 199‑234). The furin-like domains are sufficient for beta -catenin stabilization (2). Mature mouse R‑Spondin 4 shares 81%, 97%, 79%, 77% and 76% aa identity with human, rat, bovine, equine and canine R-Spondin 4, respectively. There is one potential isoform where Arg substitutes for the C‑terminal 82 amino acids (5). Each R‑Spondin has a distinct expression pattern (6). In the mouse, R‑Spondin 4 mRNA is found during development of limb bud mesenchyme, nail beds, heart and teeth (6‑8). In humans, mutations of R‑Spondin 4 have been found to cause anonychia, a condition in which fingernails and toenails are absent (8‑10).

References

Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
Kim, K.-A. et al. (2008) Mol. Biol. Cell 19:2588.
Hendrickx, M. and L. Leyns (2008) Develop. Growth Differ. 50:229.
Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
Entrez Accession # Q8BJ73, Isoform 2.
Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
Pemberton, T.J. et al. (2007) Dev. Dyn. 236:2245.
Ishii, Y. et al. (2008) J. Invest. Dermatol. 128:867.
Blaydon, D.C. et al. (2006) Nat. Genet. 38:1245.
Bergmann, C. et al. (2006) Am. J. Hum. Genet. 79:1105.

Long Name

Roof Plate-specific Spondin 4

Entrez Gene IDs

343637 (Human); 228770 (Mouse)

Alternate Names

C20orf182; chromosome 20 open reading frame 182; Cristin 4; CRISTIN4; dJ824F16.3; FLJ16018; hRspo4; Roof plate-specific spondin-4; RSPO4; RSpondin 4; R-Spondin 4; R-spondin family, member 4; R-spondin-4