Mouse Pygopus-1 Antibody

Catalog #: AF3533 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3533
AF3533-SP

All Pygopus-1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Pygopus-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Pygopus-1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Pygopus-1
Ser2-Ala417
Accession # Q9D0P5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Pygopus‑1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pygopus-1

Pygopus (the name of a legless Australian lizard) is a 45 kDa transcription co-activator that functions in the Wnt signaling pathway. Mouse Pygopus-1 is a nuclear protein that is synthesized as a 417 amino acid residue protein that has the conserved N-terminal homology domain (NHD) and the C-terminal PHD (Plant homeodomain) finger Zn²⁺-binding domain. A nuclear localization signal is present within the NHD. The PHD finger of Pygopus binds to Legless/Bcl9, which in turn binds beta -Catenin, which recruits TCF/LEF DNA binding proteins. Formation of the quaternary complex is required for Wnt signaling. Mouse and human Pygopus-1 share 87% amino acid sequence homology.

Entrez Gene IDs

26108 (Human); 72135 (Mouse)

Alternate Names

DKFZp547G0910; Pygo1; pygopus 1; pygopus homolog 1 (Drosophila); pygopus homolog 1; Pygopus1; Pygopus-1; pygopus-like protein 1