Mouse Plasminogen Kringle 5 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF742
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Mouse Plasminogen Kringle 5 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Plasminogen Kringle 5 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Angiostatin (Kringle 1‑3) is observed. This antibody is expected to show cross-reactivity with mouse Plasminogen.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Plasminogen Kringle 5
Cys481-Ala563
Accession # AAA50168
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Plasminogen Kringle 5

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plasminogen Kringle 5

Plasminogen is a coenzyme that has five triple disulfide-linked kringle domains. It is converted to plasmin by plasminogen activators. Internal fragments of plasminogen, including the naturally occurring angiostatin isoforms and Kringle 5, can also be generated by proteolytic cleavage. These internal fragments have been shown to be potent angiogenesis inhibitors (1).

Entrez Gene IDs
5340 (Human); 18815 (Mouse); 85253 (Rat)
Alternate Names
HAE4; Plasminogen Kringle 5

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