Mouse Plasminogen Kringle 5 Antibody

Catalog # Availability Size / Price Qty
MAB742
MAB742-SP
Detection of Mouse Plasminogen Kringle 5 by Western Blot
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Product Details
Citations (1)
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Mouse Plasminogen Kringle 5 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Plasminogen Kringle 5 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant human Plasminogen Kringle 5 is observed.
Source
Monoclonal Rat IgG1 Clone # 120304
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Plasminogen Kringle 5
Cys481-Ala563
Accession # AAA50168
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Plasminogen Kringle 5

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Plasminogen Kringle 5 by Western Blot View Larger

Detection of Mouse Plasminogen Kringle 5 by Western Blot Ferulic acid regulated the differential expression of angiogenic and antiangiogenic factors in MN mouse model.  At the end of the experiment, kidney samples of different groups were harvested and freshly isolated the glomeruli according to the Materials and Methods. Total protein lysate was extracted from isolated glomeruli and separated in 10% SDS page by electrophoresis, and further blotting by anti-VEGF (a), TSP-1 (b), and K5 (c) antibodies. The bar chart represented the semiquantification level of the expression density (target/actin) (Left Panel) (*P < 0.05; **P < 0.01). Immunofluorescence staining was also undertaken to exam the expression level and location of VEGF, TSP-1, and K5 in NC and N-T, original magnifications, 400x (Right Panel). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22844329), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plasminogen Kringle 5

Plasminogen is a coenzyme that has five triple disulfide-linked kringle domains. It is converted to plasmin by plasminogen activators. Internal fragments of plasminogen, including the naturally occurring angiostatin isoforms and Kringle 5, can also be generated by proteolytic cleavage. These internal fragments have been shown to be potent angiogenesis inhibitors (1).

References
  1. Cao, Y. et al. (1997) J. Biol. Chem. 272:22924.
Entrez Gene IDs
5340 (Human); 18815 (Mouse); 85253 (Rat)
Alternate Names
HAE4; Plasminogen Kringle 5

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Citation for Mouse Plasminogen Kringle 5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Histone H2B as a functionally important plasminogen receptor on macrophages.
    Authors: Das R, Burke T, Plow EF
    Blood, 2007-08-09;110(10):3763-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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