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Mouse Osteoactivin/GPNMB Antibody

Catalog #: AF2330
28 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2330
AF2330-SP
Cell Adhesion Mediated by Osteoactivin/GPNMB and Neutralization by Mouse Osteoactivin/GPNMB Antibody.
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Citations (28)
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Mouse Osteoactivin/GPNMB Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Osteoactivin/GPNMB in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) Osteoactivin, rhSyndecan-4, and recombinant mouse Syndecan-4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Osteoactivin/GPNMB
Lys23-Asn502
Accession # Q8BVA0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (Catalog # 2330-AC)

Mouse Osteoactivin/GPNMB Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Osteoactivin/GPNMB Biotinylated Antibody (Catalog # BAF2330)

Standard: Recombinant Mouse Osteoactivin/GPNMB Fc Chimera Protein, CF (Catalog # 2330-AC)

Neutralization
Measured by its ability to neutralize Osteoactivin/GPNMB-mediated adhesion of the SVEC4‑10 mouse vascular endothelial cell line. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 0.25 µg/mL Recombinant Mouse Osteoactivin/GPNMB Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Adhesion Mediated by Osteoactivin/GPNMB and Neutralization by Mouse Osteoactivin/GPNMB Antibody. View Larger

Cell Adhesion Mediated by Osteoactivin/GPNMB and Neutralization by Mouse Osteoactivin/GPNMB Antibody. Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (Catalog # 2330-AC), immobilized onto a microplate previously coated with Goat Anti-Mouse IgG Fc (Catalog # G-202-C), supports the adhesion of the SVEC4-10 mouse vascular endothelial cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Osteoactivin/ GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2330). The ND50 is typically 0.2-0.8 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteoactivin/GPNMB

Osteoactivin (also named GPNMB and DC-HIL) is a 125 kDa, intracellular glycoprotein that is associated with cell endosomal/lysosomal compartments (1, 2). Mouse osteoactivin is synthesized as a type I, transmembrane, 574 amino acid (aa) precursor that contains a 22 aa signal sequence, a 478 aa luminal/extracellular domain, a 23 aa transmembrane segment and a 51 aa cytoplasmic tail. The luminal region contains an N-terminal heparin-binding motif, multiple glycosylation sites, an RGD motif and a 130 aa PKD domain. The intracellular tail also has an RGD motif, plus an ITAM (Y-x-x-I) and lysosomal targeting (L-L) motif. The extracellular/luminal region is 89% and 74% aa identical to the equivalent regions in rat and human, respectively. Cells known to express osteoactivin include osteoblasts, dendritic cells, and melanocytes, plus fetal chondrocytes and stratum basale keratinocytes (2, 3). Osteoactivin is reported to bind to heparan sulfate-proteoglycan, possibly on the surface of fibroblasts and endothelial cells (2). It may also interact with integrins.

References
  1. Bachner, D. et al. (2002) Gene Exp. Patterns 1:159.
  2. Shikano, S. et al. (2001) J. Biol. Chem. 276:8125.
  3. Owen, T.A. et al. (2003) Crit. Rev. Eukaryot. Gene Expr. 13:205.
Long Name
Glycoprotein Non-Metastatic Melanoma Protein B
Entrez Gene IDs
10457 (Human); 93695 (Mouse); 113955 (Rat); 102133517 (Cynomolgus Monkey)
Alternate Names
DC-HIL; glycoprotein (transmembrane) nmb; glycoprotein NMB; GPNMB; HGFIN; HGFINglycoprotein nmb-like protein; NMBosteoactivin; Osteoactivin; Transmembrane glycoprotein HGFIN; transmembrane glycoprotein NMB

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Citations for Mouse Osteoactivin/GPNMB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. The potential of GPNMB as novel neuroprotective factor in amyotrophic lateral sclerosis
    Authors: Hirotaka Tanaka, Masamitsu Shimazawa, Masataka Kimura, Masafumi Takata, Kazuhiro Tsuruma, Mitsunori Yamada et al.
    Scientific Reports
  2. Loss of TMEM106B and PGRN leads to severe lysosomal abnormalities and neurodegeneration in mice
    Authors: Feng T, Mai S, Roscoe JM et al.
    EMBO Rep.
  3. Deconstructing cold-induced brown adipocyte neogenesis in mice
    Authors: Rayanne B Burl, Elizabeth Ann Rondini, Hongguang Wei, Roger Pique-Regi, James G Granneman
    eLife
  4. Progranulin deficiency results in sex-dependent alterations in microglia in response to demyelination
    Authors: Zhang T, Feng T, Wu K et al.
    Acta Neuropathologica
  5. A multifaceted role of progranulin in regulating amyloid-beta dynamics and responses
    Authors: Du H, Wong MY, Zhang T et al.
    Life science alliance
  6. Epistatic interaction between the lipase-encoding genes Pnpla2 and Lipe causes liposarcoma in mice
    Authors: Jiang Wei Wu, Christoph Preuss, Shu Pei Wang, Hao Yang, Bo Ji, Gregory W. Carter et al.
    PLOS Genetics
  7. Tumor associated microglia/macrophages utilize GPNMB to promote tumor growth and alter immune cell infiltration in glioma
    Authors: Yalcin, F;Haneke, H;Efe, IE;Kuhrt, LD;Motta, E;Nickl, B;Flüh, C;Synowitz, M;Dzaye, O;Bader, M;Kettenmann, H;
    Acta neuropathologica communications
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Mimicking angiogenic microenvironment of alveolar soft-part sarcoma in a microfluidic coculture vasculature chip
    Authors: Chuaychob, S;Lyu, R;Tanaka, M;Haginiwa, A;Kitada, A;Nakamura, T;Yokokawa, R;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Mouse
    Sample Types: Spheroid
    Applications: Immunohistochemistry
  9. Border-associated macrophages mediate the neuroinflammatory response in an alpha-synuclein model of Parkinson disease
    Authors: Schonhoff, AM;Figge, DA;Williams, GP;Jurkuvenaite, A;Gallups, NJ;Childers, GM;Webster, JM;Standaert, DG;Goldman, JE;Harms, AS;
    Nature communications
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Integrated single-cell transcriptomic analyses reveal that GPNMB-high macrophages promote PN-MES transition and impede T cell activation in GBM
    Authors: A Xiong, J Zhang, Y Chen, Y Zhang, F Yang
    EBioMedicine, 2022-08-30;83(0):104239.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Glycoprotein nonmetastatic melanoma protein B regulates lysosomal integrity and lifespan of senescent cells
    Authors: M Suda, I Shimizu, G Katsuumi, CL Hsiao, Y Yoshida, N Matsumoto, Y Yoshida, A Katayama, J Wada, M Seki, Y Suzuki, S Okuda, K Ozaki, M Nakanishi-, T Minamino
    Scientific Reports, 2022-04-20;12(1):6522.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Serotonin transporter-mediated molecular axis regulates regional retinal ganglion cell vulnerability and axon regeneration after nerve injury
    Authors: R Kingston, D Amin, S Misra, JM Gross, T Kuwajima
    PloS Genetics, 2021-11-04;17(11):e1009885.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. GPNMB plays a protective role against obesity-related metabolic disorders by reducing macrophage inflammatory capacity
    Authors: A Prabata, K Ikeda, EP Rahardini, KI Hirata, N Emoto
    The Journal of Biological Chemistry, 2021-09-25;0(0):101232.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Quantitative trait locus mapping identifies the Gpnmb gene as a modifier of mouse macrophage lysosome function
    Authors: P Robinet, B Ritchey, SW Lorkowski, AM Alzayed, S DeGeorgia, E Schodowski, CA Traughber, JD Smith
    Scientific Reports, 2021-05-13;11(1):10249.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. GCase and LIMP2 Abnormalities in the Liver of Niemann Pick Type C Mice
    Authors: MJC van der Li, J Aten, ARA Marques, ISE Waas, PWB Larsen, N Claessen, NN van der We, R Ottenhoff, M van Eijk, JMFG Aerts
    International Journal of Molecular Sciences, 2021-03-03;22(5):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Western Blot
  16. Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations
    Authors: M Huang, E Modeste, E Dammer, P Merino, G Taylor, DM Duong, Q Deng, CJ Holler, M Gearing, D Dickson, NT Seyfried, T Kukar
    Acta Neuropathol Commun, 2020-10-07;8(1):163.
    Species: Mouse
    Sample Types: Protein, Whole Tissue
    Applications: IHC-P, Western Blot
  17. Substrate Reduction Therapy for Sandhoff Disease through Inhibition of Glucosylceramide Synthase Activity
    Authors: J Marshall, JB Nietupski, H Park, J Cao, DS Bangari, C Silvescu, T Wilper, K Randall, D Tietz, B Wang, X Ying, JP Leonard, SH Cheng
    Mol. Ther., 2019-06-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Glycoprotein NMB: a novel Alzheimer's disease associated marker expressed in a subset of activated microglia
    Authors: M Hüttenrauc, I Ogorek, H Klafki, M Otto, C Stadelmann, S Weggen, J Wiltfang, O Wirths
    Acta Neuropathol Commun, 2018-10-19;6(1):108.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. HEPES activates a MiT/TFE-dependent lysosomal-autophagic gene network in cultured cells: A call for caution
    Authors: MJ Tol, MJC van der Li, TL Gabriel, JJ Hagen, S Scheij, T Veenendaal, J Klumperman, WE Donker-Koo, AJ Verhoeven, H Overkleeft, JM Aerts, CA Argmann, M van Eijk
    Autophagy, 2018-02-17;0(0):1-13.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Glycoprotein nonmetastatic melanoma protein B extracellular fragment shows neuroprotective effects and activates the PI3K/Akt and MEK/ERK pathways via the Na+/K+-ATPase.
    Authors: Yoko Ono, Kazuhiro Tsuruma, Masafumi Takata, Masamitsu Shimazawa, Hideaki Hara
    Scientific Reports, 2016-03-18;0(0):2045-2322.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Gpnmb Is a Potential Marker for the Visceral Pathology in Niemann-Pick Type C Disease.
    Authors: Marques A, Gabriel T, Aten J, van Roomen C, Ottenhoff R, Claessen N, Alfonso P, Irun P, Giraldo P, Aerts J, van Eijk M
    PLoS ONE, 2016-01-15;11(1):e0147208.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  22. A role for Na+,K+-ATPase alpha1 in regulating Rab27a localisation on melanosomes.
    Authors: Booth A, Tarafder A, Hume A, Recchi C, Seabra M
    PLoS ONE, 2014-07-22;9(7):e102851.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Lysosomal stress in obese adipose tissue macrophages contributes to MITF-dependent Gpnmb induction.
    Authors: Gabriel T, Tol M, Ottenhof R, van Roomen C, Aten J, Claessen N, Hooibrink B, de Weijer B, Serlie M, Argmann C, van Elsenburg L, Aerts J, van Eijk M
    Diabetes, 2014-05-01;63(10):3310-23.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  24. Osteoactivin is a novel osteoclastic protein and plays a key role in osteoclast differentiation and activity.
    Authors: Sheng MH, Wergedal JE, Mohan S, Lau KH
    FEBS Lett., 2008-03-31;582(10):1451-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  25. Dependency of intraocular pressure elevation and glaucomatous changes in DBA/2J and DBA/2J-Rj mice.
    Authors: Scholz M, Buder T, Seeber S, Adamek E, Becker CM, Lutjen-Drecoll E
    Invest. Ophthalmol. Vis. Sci., 2008-02-01;49(2):613-21.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  26. The glycoprotein GPNMB is selectively elevated in the substantia nigra of Parkinson's disease patients and increases after lysosomal stress
    Authors: Elizabeth B. Moloney, Alyssa Moskites, Eliza J. Ferrari, Ole Isacson, Penelope J. Hallett
    Neurobiology of Disease
  27. The novel monoclonal antibody 9F5 reveals expression of a fragment of GPNMB/osteoactivin processed by furin‐like protease(s) in a subpopulation of microglia in neonatal rat brain
    Authors: Kohichi Kawahara, Hiroshi Hirata, Kengo Ohbuchi, Kentaro Nishi, Akira Maeda, Akihiko Kuniyasu et al.
    Glia
  28. Expression of glycoprotein nonmetastatic melanoma protein B in macrophages infiltrating injured mucosa is associated with the severity of experimental colitis in mice
    Authors: FUMISATO SASAKI, KOTARO KUMAGAI, HIROFUMI UTO, YOICHIRO TAKAMI, TAKESHI KURE, KAZUAKI TABU et al.
    Molecular Medicine Reports

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