Mouse Osteoactivin/GPNMB Antibody

Catalog #: MAB2330 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2330
MAB2330-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Osteoactivin/GPNMB Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Osteoactivin/GPNMB in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human Osteoactivin.

Source

Monoclonal Rat IgG_2B Clone # 297310

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Osteoactivin/GPNMB
Lys23-Asn502
Accession # NP_444340

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (Catalog # 2330-AC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteoactivin/GPNMB

Osteoactivin (also named GPNMB and DC-HIL) is a 125 kDa, intracellular glycoprotein that is associated with cell endosomal/lysosomal compartments (1, 2). Mouse osteoactivin is synthesized as a type I, transmembrane, 574 amino acid (aa) precursor that contains a 22 aa signal sequence, a 478 aa luminal/extracellular domain, a 23 aa transmembrane segment and a 51 aa cytoplasmic tail. The luminal region contains an N-terminal heparin-binding motif, multiple glycosylation sites, an RGD motif and a 130 aa PKD domain. The intracellular tail also has an RGD motif, plus an ITAM (Y-x-x-I) and lysosomal targeting (L-L) motif. The extracellular/luminal region is 89% and 74% aa identical to the equivalent regions in rat and human, respectively. Cells known to express osteoactivin include osteoblasts, dendritic cells, and melanocytes, plus fetal chondrocytes and stratum basale keratinocytes (2, 3). Osteoactivin is reported to bind to heparan sulfate-proteoglycan, possibly on the surface of fibroblasts and endothelial cells (2). It may also interact with integrins.

References

Bachner, D. et al. (2002) Gene Exp. Patterns 1:159.
Shikano, S. et al. (2001) J. Biol. Chem. 276:8125.
Owen, T.A. et al. (2003) Crit. Rev. Eukaryot. Gene Expr. 13:205.

Long Name

Glycoprotein Non-Metastatic Melanoma Protein B

Entrez Gene IDs

10457 (Human); 93695 (Mouse); 113955 (Rat); 102133517 (Cynomolgus Monkey)

Alternate Names

DC-HIL; glycoprotein (transmembrane) nmb; glycoprotein NMB; GPNMB; HGFIN; HGFINglycoprotein nmb-like protein; NMBosteoactivin; Osteoactivin; Transmembrane glycoprotein HGFIN; transmembrane glycoprotein NMB