Mouse Oncostatin M/OSM Antibody

Catalog #: MAB495
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB495
MAB495-SP

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Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Oncostatin M/OSM Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse OSM in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant hhuman (rh) OSM, rhCLC, recombinant mouse (rm) CT-1, rmIL-6, rmIL-11, rmLIF, or recombinant rat CNTF.

Source

Monoclonal Rat IgG_2A Clone # 157210

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse OSM
Ala24-Arg206
Accession # P53347.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Oncostatin M/OSM (Catalog # 495-MO)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Oncostatin M/OSM

Oncostatin M (OSM) is a member of a cytokine subfamily that includes IL-6, IL-11, LIF, CNTF, and cardiotrophin-1. These cytokines have overlapping biological functions and shared receptor components. Mouse OSM was cloned and identified as an immediate early gene induced in various myeloid and lymphoid cell lines by a subset of cytokines including IL-2, IL-3, GM-CSF, and Erythropoietin. The mouse OSM cDNA encodes a 263 amino acid residue precursor protein that shows 48% identity with human OSM. Similar to human OSM, the C-terminal region of mouse OSM contains a highly charged region. Deletion of this C-terminal region appears to be essential for the formation of biologically active mouse OSM. The biological activity of human OSM has been shown to be mediated either by the LIF/OSM receptor complex composed of gp130 and LIF R alpha or by a human OSM specific receptor composed of gp130 and OSM R alpha. It remains to be determined if the biological activities of mouse OSM can also be mediated by both receptor complexes in mouse cells.

References

Yoshimura, A. et al. (1996) EMBO J. 15:1055.
Ray, P. et al. (1996) Endocrinology 137:1151.
Rose, T.M. and A.G. Bruce (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, editor, Oxford University Press, New York, p. 127.

Entrez Gene IDs

5008 (Human); 18413 (Mouse)

Alternate Names

MGC20461; oncostatin M; oncostatin-M; OSM

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Product Datasheet

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Citation for Mouse Oncostatin M/OSM Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1