Mouse NKG2A/CD159a Antibody

Catalog #: MAB6867 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6867
MAB6867-SP

Detection of NKG2A in HEK293 Human Cell Line transfected with Mouse NKG2A and eGFP by Flow Cytometry

2 Images

All NKG2A/CD159a Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse NKG2A/CD159a Antibody Summary

Species Reactivity

Mouse

Specificity

Stains mouse NKG2A/CD159a transfectants and not irrelevant transfectants in flow cytometry.

Source

Monoclonal Rat IgG_2B Clone # 705829

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

CHO Chinese hamster ovary cell line transfected with mouse NKG2A/CD159a
Accession # Q9Z202

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of NKG2A in HEK293 Human Cell Line transfected with Mouse NKG2A and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with either (A) mouse NKG2A or (B) irrelevant protein, and eGFP, was stained with Rat anti-mouse NKG2A monoclonal antibody (Catalog # MAB6867) followed by Allophycocyanin-conjugated anti-Rat IgG Secondary Antibody (F0113). Quadrant markers were set based on control antibody staining (MAB0061, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Flow Cytometry

View Larger

Detection of NKG2A/CD159a in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse NKG2A/CD159a Monoclonal Antibody (Catalog # MAB6867) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113) and Rat Anti-Mouse Integrin a2/CD49b PE-conjugated Monoclonal Antibody (Catalog # FAB1740P). Quadrant markers were set based on control antibody staining (Catalog # MAB0061).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2A/CD159a

NKG2A, also known as CD159a, is a 244 amino acid (aa), ~38 kDa type II transmembrane glycoprotein with a cytoplasmic ITIM motif and a single extracellular lectin-like domain. Within the extracellular domain (aa 94-244), mouse NKG2A shares 71% and 41% aa sequence identity with rat and human NKG2A, respectively. An isoform lacking aa 98-114 has been described. NKG2A associates with CD94 and is coexpressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its murine ligand, Qa-1b (or HLA-E in humans) on antigen presenting cells.

Long Name

Natural Killer G2A

Entrez Gene IDs

3821 (Human); 16641 (Mouse); 102121106 (Cynomolgus Monkey)

Alternate Names

CD159 antigen-like family member A; CD159a antigen; CD159a; C-lectin type II protein; killer cell lectin-like receptor subfamily C, member 1; Klrc1; MGC13374; natural killer cell lectin; natural killer group protein 2; NK cell receptor A; NKG2; NKG2-1/B activating NK receptor; NKG2A; NKG2-A; NKG2-A/B type II integral membrane protein; NKG2-A/B-activating NK receptor; NKG2-A/NKG2-B type II integral membrane protein; NKG2AMGC59791; NKG2-B