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Mouse NG2/MCSP Antibody

Catalog #: MAB6689
14 Citations 1 Review Datasheet / COA / SDS
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MAB6689
MAB6689-SP
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NG2/MCSP in Mouse Brain.
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Citations (14)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse NG2/MCSP Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse NG2/MCSP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human NG2/MCSP/CSPG4 is observed.
Source
Monoclonal Rat IgG1 Clone # 546930
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse NG2/MCSP
Leu1587-Ser2229
Accession # Q8VHY0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry NG2/MCSP antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

NG2/MCSP in Mouse Brain. NG2/MCSP was detected in perfusion fixed frozen sections of mouse brain (cortex) using Rat Anti-Mouse NG2/MCSP Monoclonal Antibody (Catalog # MAB6689) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hemotoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NG2/MCSP

NG2, also called MCSP (melanoma-associated chondroitin sulfate proteoglycan), CSPG4, or AN2, is a 400-500 kDa proteoglycan found on multiple cell types, including immature oligodendrocytes, osteoblasts, chondroblasts, and pericytes.  It interacts with integrin alpha 3 beta 1, galectin 3, MMP16, collagen VI and PDGFR alpha.  Mature mouse NG2 is a 2298 amino acid (aa) type I transmembrane glycoprotein.  The extracellular region is 2200 aa in length (aa 30-2229), while the cytoplasmic domain is 76 aa in size. The glycoprotein component of NG2 is approximately 300-315 kDa in size.  Proteolytic cleavage generates a 290 and 275 kDa form (excluding the chondroitin sulfate moiety).  There are two splice variants.  One shows a 38 aa substitution for aa 1657-2327, while another shows an alternate start site at Met1667.  Over aa 31-420, mouse NG2 shares 95% and 80% aa identity with rat and human NG2, respectively.    

Long Name
Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan
Entrez Gene IDs
1464 (Human); 121021 (Mouse); 81651 (Rat)
Alternate Names
AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8

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Citations for Mouse NG2/MCSP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Identification of distinct ChAT(+) neurons and activity-dependent control of postnatal SVZ neurogenesis.
    Authors: Paez-Gonzalez Patricia, Asrican Brent, Rodriguez Erica, Kuo Chay T.
    Nat Neurosci.
  2. Runx1+ vascular smooth muscle cells are essential for hematopoietic stem and progenitor cell development in vivo
    Authors: Gonzalez Galofre, ZN;Kilpatrick, AM;Marques, M;Sá da Bandeira, D;Ventura, T;Gomez Salazar, M;Bouilleau, L;Marc, Y;Barbosa, AB;Rossi, F;Beltran, M;van de Werken, HJG;van IJcken, WFJ;Henderson, NC;Forbes, SJ;Crisan, M;
    Nature communications
    Species: Mouse
    Sample Types: Embryo
    Applications: IHC
  3. 20-?Hydroxycholesterol, an oxysterol in human breast milk, reverses mouse neonatal white matter injury through Gli-dependent oligodendrogenesis
    Authors: Chao, AS;Matak, P;Pegram, K;Powers, J;Hutson, C;Jo, R;Dubois, L;Thompson, JW;Smith, PB;Jain, V;Liu, C;Younge, NE;Rikard, B;Reyes, EY;Shinohara, ML;Gregory, SG;Goldberg, RN;Benner, EJ;
    Cell stem cell
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. NO-sensitive guanylyl cyclase discriminates pericyte-derived interstitial from intra-alveolar myofibroblasts in murine pulmonary fibrosis
    Authors: Aue, A;Englert, N;Harrer, L;Schwiering, F;Gaab, A;König, P;Adams, R;Schmidtko, A;Friebe, A;Groneberg, D;
    Respiratory research
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Active immunotherapy reduces NOTCH3 deposition in brain capillaries in a CADASIL mouse model
    Authors: DV Oliveira, KG Coupland, W Shao, S Jin, F Del Gaudio, S Wang, R Fox, JW Rutten, J Sandin, H Zetterberg, J Lundkvist, SA Lesnik Obe, U Lendahl, H Karlström
    Embo Molecular Medicine, 2022-12-16;0(0):e16556.
    Species: Mouse
    Sample Types: Tissue Homeogenates
    Applications: IHC
  6. The alarmin interleukin-1? triggers secondary degeneration through reactive astrocytes and endothelium after spinal cord injury
    Authors: Bretheau F, Castellanos-Molina A, B�langer D et al.
    Nature Communications
  7. PDGFRbeta+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny
    Authors: D Sá da Band, AM Kilpatrick, M Marques, M Gomez-Sala, T Ventura, ZN Gonzalez, D Stefancova, F Rossi, M Vermeren, CS Vink, M Beltran, NC Henderson, B Jung, R van der Li, HJG van de Wer, WFJ van Ijcken, C Betsholtz, SJ Forbes, H Cuervo, M Crisan
    Oncogene, 2022-07-19;40(3):111114.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Deficiency of Notch signaling in pericytes results in arteriovenous malformations
    Authors: T Nadeem, W Bogue, B Bigit, H Cuervo
    JCI Insight, 2020-11-05;5(21):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Suppression of proteolipid protein rescues Pelizaeus–Merzbacher disease
    Authors: Matthew S. Elitt, Lilianne Barbar, H. Elizabeth Shick, Berit E. Powers, Yuka Maeno-Hikichi, Mayur Madhavan et al.
    Nature
  10. Dysregulated mesenchymal PDGFR-&beta drives kidney fibrosis
    Authors: EM Buhl, S Djudjaj, BM Klinkhamme, K Ermert, VG Puelles, MT Lindenmeye, CD Cohen, C He, E Borkham-Ka, R Weiskirche, B Denecke, P Trairatphi, J Saez-Rodri, TB Huber, LE Olson, J Floege, P Boor
    EMBO Mol Med, 2020-01-14;0(0):e11021.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Metformin inhibits metastatic breast cancer progression and improves chemosensitivity by inducing vessel normalization via PDGF-B downregulation
    Authors: JC Wang, GY Li, B Wang, SX Han, X Sun, YN Jiang, YW Shen, C Zhou, J Feng, SY Lu, JL Liu, MD Wang, PJ Liu
    J. Exp. Clin. Cancer Res., 2019-06-04;38(1):235.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. O-GlcNAc on NOTCH1 EGF repeats regulates ligand-induced Notch signaling and vascular development in mammals
    Authors: Sawaguchi S, Varshney S, Ogawa M et al.
    eLife
  13. Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy
    Authors: N Yang, W Zhang, T He, Y Xing
    Exp. Eye Res, 2017-02-28;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  14. Equine-Induced Pluripotent Stem Cells Retain Lineage Commitment Toward Myogenic and Chondrogenic Fates
    Authors: Mattia Quattrocelli, Giorgia Giacomazzi, Sarah Y. Broeckx, Liesbeth Ceelen, Selin Bolca, Jan H. Spaas et al.
    Stem Cell Reports

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Reviews for Mouse NG2/MCSP Antibody

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Mouse NG2/MCSP Antibody
By Anonymous on 11/02/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Brain tissue Species: Mouse
Immunocytochemistry/Immunofluorescence Mouse NG2/MCSP Antibody MAB6689