Mouse NCAM-1/CD56 APC-conjugated Antibody Summary
Leu20-Thr711
Accession # P13595
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of NCAM‑1/CD56 in Neuro‑2A Mouse Cell Line by Flow Cytometry. Neuro-2A mouse neuroblastoma cell line was stained with Rat Anti-Mouse NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB7820A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NCAM-1/CD56
NCAM-1 (Neural adhesion molecule-1; also CD56) is a 120-190 kDa glycoprotein member of the Ig Superfamily. It is expressed on multiple cell types, both in the embryo and adult. Here, it serves as both an adhesion molecule and a receptor for multiple ligands, including as FGFR, PDGF, GDNF and agrin. On the cell surface, it is a cis-oriented homodimer that can form homodimers in-trans with other cis-homodimers. In the embryo, NCAM-1 is polysialylated (PolySia), and shows a MW of 200-220 kDa in SDS-PAGE. This polysialylation reduces the ability of NCAM-1 to dimerize. Mature mouse NCAM-1 is a 1096 amino acid (aa) type I transmembrane (TM) protein (aa 20-1115). It possesses a 692 aa extracellular region (aa 20-711) and a 386 aa cytoplasmic domain. The extracellular region contains five consecutive C2-type Ig-like domains (aa 20-492) followed by two FN type-III domains (aa 497-692). Multiple splice variants exist. There is a 140 kDa TM variant that shows a deletion of aa 810-1076, and a 120 kDa variant that is GPI-linked and shows a 24 aa substitution for aa 702-1115. A third potential variant contains a five aa substitution for aa 601-1115. Over aa 20-711, mouse NCAM-1 shares 99% and 95% aa identity with rat and human NCAM-1, respectively.
Product Datasheets
Citations for Mouse NCAM-1/CD56 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Clearance of senescent glial cells prevents tau-dependent pathology and cognitive decline
Authors: TJ Bussian, A Aziz, CF Meyer, BL Swenson, JM van Deurse, DJ Baker
Nature, 2018-09-19;0(0):.
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Blocking the angiopoietin-2-dependent integrin ?-1 signaling axis abrogates small cell lung cancer invasion and metastasis
Authors: Meder, L;Orschel, CI;Otto, CJ;Koker, M;Brägelmann, J;Ercanoglu, MS;Dähling, S;Compes, A;Selenz, C;Nill, M;Dietlein, F;Florin, A;Eich, ML;Borchmann, S;Odenthal, M;Blazquez, R;Hilberg, F;Klein, F;Hallek, M;Büttner, R;Reinhardt, HC;Ullrich, RT;
JCI insight
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
In vivo study of the immune response to bioengineered spider silk spheres
Authors: T Deptuch, K Penderecka, M Kaczmarek, S Molenda, H Dams-Kozlo
Scientific Reports, 2022-08-05;12(1):13480.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
Authors: YT Shue, AP Drainas, NY Li, SM Pearsall, D Morgan, N Sinnott-Ar, SQ Hipkins, GL Coles, JS Lim, AE Oro, KL Simpson, C Dive, J Sage
Nature Communications, 2022-05-16;13(1):2690.
Species: Mouse
Sample Types: Whole Tissue
Applications: Flow Cytometry -
STRAP regulates alternative splicing fidelity during lineage commitment of mouse embryonic stem cells
Authors: L Jin, Y Chen, DK Crossman, A Datta, T Vu, JA Mobley, MK Basu, M Scarduzio, H Wang, C Chang, PK Datta
Nat Commun, 2020-11-23;11(1):5941.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Oncolytic virotherapy for small-cell lung cancer induces immune infiltration and prolongs survival
Authors: P Kellish, D Shabashvil, MM Rahman, A Nawab, MV Guijarro, M Zhang, C Cao, N Moussatche, T Boyle, S Antonia, M Reinhard, C Hartzell, M Jantz, HJ Mehta, G McFadden, FJ Kaye, M Zajac-Kaye
J. Clin. Invest., 2019-04-29;130(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
CNS-derived extracellular vesicles from superoxide dismutase 1 (SOD1)G93A ALS mice originate from astrocytes and neurons and carry misfolded SOD1
Authors: JM Silverman, D Christy, CC Shyu, KM Moon, S Fernando, Z Gidden, CM Cowan, Y Ban, RG Stacey, LI Grad, L McAlary, IR Mackenzie, LJ Foster, NR Cashman
J. Biol. Chem., 2019-01-11;0(0):.
Species: Mouse
Sample Types: Microvesicles
Applications: Flow Cytometry -
GDF10 is a signal for axonal sprouting and functional recovery after stroke.
Authors: Li S, Nie E, Yin Y, Benowitz L, Tung S, Vinters H, Bahjat F, Stenzel-Poore M, Kawaguchi R, Coppola G, Carmichael S
Nat Neurosci, 2015-10-26;18(12):1737-45.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Flow Cytometry -
Neuronal transgene expression in dominant-negative SNARE mice.
Authors: Fujita T, Chen M, Li B, Smith N, Peng W, Sun W, Toner M, Kress B, Wang L, Benraiss A, Takano T, Wang S, Nedergaard M
J Neurosci, 2014-12-10;34(50):16594-604.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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