Mouse MICL/CLEC12A Antibody

Catalog #: AF2950
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2950
AF2950-SP

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All MICL/CLEC12A Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse MICL/CLEC12A Antibody Summary

Species Reactivity

Mouse

Specificity

Detects recombinant mouse MICL/CLEC12A in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
1% cross‑reactivity with recombinant human MICL is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MICL/CLEC12A
Thr67-Arg267
Accession # NP_808354

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse MICL/CLEC12A

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MICL/CLEC12A

MICL, also known as killer cell lectin-like receptor 1 (KLR1), is an inhibitory C-type lectin-like receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM). It is a75 kDa type II membrane protein that is expressed on neutrophils, NK cells, monocytes and dendritic cells. The amino acid sequence of mouse MICL extracellular domain is 53%, 52% and 58% identical to that of human, bovine and canine MICL, respectively.

Long Name

Myeloid Inhibitory C-type Lectin-like Receptor

Entrez Gene IDs

160364 (Human); 232413 (Mouse)

Alternate Names

CD371; CLEC12A; CLL1; CLL-1; C-type lectin domain family 12, member A; DCAL-2; MICL

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse MICL/CLEC12A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Clec12a inhibits MSU-induced immune activation through lipid raft expulsion
Authors: Ying Xu, Dingka Song, Wei Wang, Shixin Li, Tongtao Yue, Tie Xia et al.
Life Science Alliance
Clec12a is an inhibitory receptor for uric acid crystals that regulates inflammation in response to cell death.
Authors: Neumann K, Castineiras-Vilarino M, Hockendorf U, Hannesschlager N, Lemeer S, Kupka D, Meyermann S, Lech M, Anders H, Kuster B, Busch D, Gewies A, Naumann R, Gross O, Ruland J
Immunity, 2014-03-13;40(3):389-99.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot