Mouse MICL/CLEC12A Antibody

Catalog #: MAB2950 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2950-100
MAB2950-SP

Detection of MICL/CLEC12A in Mouse Splenocytes by Flow Cytometry.

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All MICL/CLEC12A Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse MICL/CLEC12A Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse MICL/CLEC12A in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 812303

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MICL/CLEC12A with an N-terminal 9-His tag
Thr67-Arg267
Accession # Q504P2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MICL/CLEC12A in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with either (A) Rat Anti-Mouse MICL/CLEC12A Monoclonal Antibody (Catalog # MAB2950) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Goat Anti-Rat PE-conjugated secondary antibody (Catalog # F0105B) and Rat Anti-Mouse CD11c APC-conjugated Monoclonal Antibody (Catalog # FAB69501A). Cells were first gated on Rat Anti-Mouse CD8 alpha Alexa Fluor® 488-conjugated Monoclonal Antibody-positive cells (Catalog # FAB116G, not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MICL/CLEC12A

MICL, also known as killer cell lectin-like receptor 1 (KLR1), is an inhibitory C-type lectin-like receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM). It is a75 kDa type II membrane protein that is expressed on neutrophils, NK cells, monocytes and dendritic cells. The amino acid sequence of mouse MICL extracellular domain is 53%, 52% and 58% identical to that of human, bovine and canine MICL, respectively.

Long Name

Myeloid Inhibitory C-type Lectin-like Receptor

Entrez Gene IDs

160364 (Human); 232413 (Mouse)

Alternate Names

CD371; CLEC12A; CLL1; CLL-1; C-type lectin domain family 12, member A; DCAL-2; MICL